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TSPAN8/TM4SF3 Antibody (458811) [Unconjugated]

Images

 
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody ...read more
HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by ...read more
TSPAN8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
TSPAN8 was detected in immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
458811
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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TSPAN8/TM4SF3 Antibody (458811) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human TSPAN8
Met1-Lys237 (Ile35Val)
Accession # AAH05246
Specificity
Detects human TSPAN8 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TSPAN8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB4734 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN8/TM4SF3 Antibody (458811) [Unconjugated]

  • CO-029
  • tetraspanin 8
  • TM4SF3
  • TM4SF3tspan-8
  • Transmembrane 4 superfamily member 3tetraspanin-8
  • TSPAN8
  • Tspan-8
  • Tumor-associated antigen CO-029

Background

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta 1 Integrins, Integrin alpha 4 beta 6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSPAN8/TM4SF3 Antibody (MAB4734)(4)

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Bioinformatics

Gene Symbol TSPAN8
Uniprot