![Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-288202414191528.jpg "Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | C4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human Villin 1 aa 600-827. (Uniprot: P09327) |
| Localization | Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | VIL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Villin 1 Antibody (NBP3-33090)Find related products by research area.
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| Gene Symbol | VIL1 |
![Immunohistochemistry: Villin 1 Antibody (C4) [NBP3-33090] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33090) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-29820241416187.jpg "Immunohistochemistry: Villin 1 Antibody (C4) [NBP3-33090] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33090) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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