Novus Biologicals products are now on bio-techne.com

VAMP4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: VAMP4 Antibody [NBP2-13512] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human skeletal muscle shows weak positivity in myocytes.
Orthogonal Strategies: Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding VAMP4 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

VAMP4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDK
Marker
Vesicle Marker, Golgi Apparatus Marker
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAMP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VAMP4 Protein (NBP2-13512PEP)
Publications
Read Publication using NBP2-13512.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VAMP4 Antibody

  • VAMP24
  • VAMP-4
  • vesicle-associated membrane protein 4

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer®s disease, VAMP levels of all isoforms appear to be significantly lowered.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00010228-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NB300-510
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, WB
NBP2-00795
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB300-595
Species: Bv, Mu, Pm, Rt
Applications: Flow, IF, IHC, IHC-P, IP, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NBP2-20434
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MAB6117
Species: Hu
Applications: IHC, WB
NBP1-92467
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-87439
Species: Hu
Applications: IHC, IHC-P, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-49533
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
AF5687
Species: Hu, Rt
Applications: ICC, IHC, Simple Western, WB
NBP2-46045
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00079026-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NBP1-80883
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-45413
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-49643
Species: Bv, Hu, Pm
Applications: Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, KO, WB
NBP2-13512
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC

Publications for VAMP4 Antibody (NBP2-13512)(1)

Reviews for VAMP4 Antibody (NBP2-13512) (0)

There are no reviews for VAMP4 Antibody (NBP2-13512). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for VAMP4 Antibody (NBP2-13512) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VAMP4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol VAMP4