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VAMP-7 Antibody (549115)

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Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # ...read more
Genetic Strategies: Amphisome/lysosome fusion is not required for autophagy-mediated exosomal secretion of ANXA2. (a) Cells with VAMP7 knockdown and control knockdown were transfected with a GFP-LC3 plasmid and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
549115
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price
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VAMP-7 Antibody (549115) Summary

Immunogen
E. coli-derived recombinant human VAMP‑7
Ala2-Met140
Accession # P51809
Specificity
Detects human VAMP‑7 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
VAMP7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB6117 in the following applications:

Publications
Read Publications using
MAB6117 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-7 Antibody (549115)

  • FLJ54296
  • SYBL1
  • SYBL1FLJ53762
  • synaptobrevin-like 1
  • Synaptobrevin-like protein 1
  • tetanus neurotoxin-insensitive VAMP
  • Tetanus-insensitive VAMP
  • TIVAMP
  • TI-VAMP
  • VAMP7
  • VAMP-7
  • VAMP-7FLJ53045
  • vesicle-associated membrane protein 7

Background

Vesicle-associated membrane protein 7 (VAMP-7) is a 25 kDa, widely expressed, type IV transmembrane protein and member of the synaptobrevin family. Mature human VAMP-7 consists of a 187 aa cytoplasmic domain, a 21 aa transmembrane region, and an 11 aa vesicular region. The cytoplasmic domain contains a longin domain (aa 7‑110) and a v-SNARE coiled-coil homology domain (aa 125‑185). Two splicing variants produce three isoforms for human VAMP-7. Isoform 2 has a 116 aa substitution for aa 145‑220 found in isoform 1, and isoform 3 is missing the residues corresponding to aa 28‑68 in isoform 1. Human VAMP-7 shares 99%, 97%, and 95% aa sequence identity with bovine, mouse, and rat VAMP-7, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAMP-7 Antibody (MAB6117)(4)

Review for VAMP-7 Antibody (MAB6117) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6117:
Filter by Applications
CyTOF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
CyTOF VAMP-7 MAB6117
Enlarge
5
reviewed by:
Albert Tsai
CyTOF Human 03/18/2020
View

Summary

ApplicationCyTOF
Sample Testedbone marrow,Peripheral blood
SpeciesHuman
Lotnot recorded

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VAMP-7 Antibody (MAB6117) (0)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Albert Tsai
03/18/2020
Application: CyTOF
Species: Human

Bioinformatics

Gene Symbol VAMP7
Uniprot