Syntaxin 5 Antibody [Unconjugated] Summary
Immunogen |
E. coli-derived recombinant human Syntaxin 5 isoform 2 Met55-Thr300 Accession # Q13190 |
Specificity |
Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
STX5 |
Purity Statement |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 1-15 ug/mL
- Immunohistochemistry 3-15 ug/mL
- Simple Western 25 ug/mL
- Western Blot 0.5 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.2 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Syntaxin 5 Antibody [Unconjugated]
Background
Syntaxin 5 (STX5) is a predicted 41‑42 kDa member of the syntaxin family of proteins. It is a t‑SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis‑Golgi network (a 37 kDa form) or the ER (the 46 kDa form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single‑pass transmembrane protein (very long cytoplasmic N‑terminus) that is 355 amino acids (aa) in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t‑SNARE domain (aa 263‑325) with a coiled‑coil region (aa 287‑318), and a C‑terminal transmembrane sequence. The 35 kDa short form exhibits an alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19 aa substitution for aa 303‑355. Over aa 55‑300, human STX5 shares 94% aa identity with mouse STX5.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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