How specific are the UCP1 antibodies to UCP1 than to UCP2 and 3?

Name: UCP1 Antibody
Brand: Novus Biologicals
SKU: NB100-2828
Price: 399 USD

Question

Accepted Answer

None of our UCP1 antibodies have been tested for cross-reactivity with UCP2 or UCP3, so we cannot guarantee that these antibodies will not bind to the other UCP proteins. However, we can determine the likelihood of this cross-reactivity by running sequence alignments using the immunogen sequences. NB100-2828 is the most likely to be specific for UCP1: its short immunogen sequence is shown on its datasheet, and when I run a UniProt BLAST sequence comparison for human proteins it detects a match with UCP1 (100%), but not UCP2 or UCP3. When I use MultAlin to align the immunogen sequence with the UCP2 and UCP3 protein sequences, it shows only low similarity. For the antibodies with product codes beginning H00007350, again the immunogen sequence is shown on the datasheets for the products. In this case, the immunogen sequence (shared between them) is a little longer. When I run the UniProt Blast, the results are 100% UCP1, 66% UCP3 and 58% UCP2. For NBP2-20796, the exact immunogen sequence is not available, but I have run the UniProt Blast on the amino acid range given, and the results are 100% UCP1, 59% UCP2 and 58% UCP3. Percentages in the 50s-60s suggest that the antibody probably won't cross-react, but may.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, CaSpecies Glossary
Applications	WB, ELISA, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-EQLKRELSKSRQ corresponding to C-Terminus according to NP_068605.1.
Predicted Species	Canine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	UCP1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-2828	Applications	Species
Zhou H, Chen C, Hu H et al. High-intensity interval training improves fatty infiltration in the rotator cuff through the ?3 adrenergic receptor in mice Bone & Joint Research 2023-08-01 [PMID: 37524338]
Zhou H, Wang Z, Chen C et al. Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model Orthop J Sports Med 2023-05-19 [PMID: 37223073]
Olmsted-Davis E, Mejia J, Salisbury E Et al. A Population of M2 Macrophages Associated With Bone Formation Frontiers in immunology 2021-10-12 [PMID: 34712222] (IF/IHC, Mouse)	IF/IHC	Mouse
Wu J, Bostr0m P, Sparks LM et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012-07-20 [PMID: 22796012] (IF/IHC, Mouse)	IF/IHC	Mouse
Liu W, Singh R, Choi CS et al. LDL Receptor Related Protein 6 (LRP6) regulates body fat and glucose homeostasis by modulating nutrient sensing pathways and mitochondrial energy expenditure. The Journal of Biological Chemistry. 2012-01-01 [PMID: 22232553]
Ishigaki Y, Katagiri H, Yamada T et al. Dissipating excess energy stored in the liver is a potential treatment strategy for diabetes associated with obesity. Diabetes 2005-02-01 [PMID: 15677488]

UCP1 Antibody

Species Glossary

Images

Product Details

Order Details

UCP1 Antibody Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for UCP1 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for UCP1 Antibody (NB100-2828)(6)

Reviews for UCP1 Antibody (NB100-2828) (0)

Product General Protocols

Video Protocols

FAQs for UCP1 Antibody (NB100-2828). (Showing 1 - 1 of 1 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional UCP1 Products

Research Areas for UCP1 Antibody (NB100-2828)

Blogs on UCP1

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics

Gene Symbol	UCP1
Entrez	7350(Human)
Uniprot	NP_068605.1(Human)