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UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free

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Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (rUCHL1/775)
Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (rUCHL1/775)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (rUCHL1/775) ...read more
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Formalin-fixed, paraffin-embedded Human Brain stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).
Flow Cytometry: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (rUCHL1/775)followed by Goat anti-Mouse IgG-CF488 (Blue); ...read more
Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Analysis of Human Brain tissue lysate using Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody ...read more
Protein Array: UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free [NBP2-75775] - Analysis of Protein Array containing >19,000 full-length human proteins using UCH-L1/PGP9.5 Antibody (rUCHL1/775) Z- and S- Score: ...read more

Product Details

Summary
Reactivity Hu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA
Clone
rUCHL1/775
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75775AF350
Alexa Fluor 405
NBP2-75775AF405
Alexa Fluor 488
NBP2-75775AF488
Alexa Fluor 532
NBP2-75775AF532
Alexa Fluor 594
NBP2-75775AF594
Alexa Fluor 647
NBP2-75775AF647
Alexa Fluor 700
NBP2-75775AF700
Alexa Fluor 750
NBP2-75775AF750
Allophycocyanin
NBP2-75775APC
Biotin
NBP2-75775B
CoraFluor 1
NBP2-75775CL1
DyLight 350
NBP2-75775UV
DyLight 405
NBP2-75775V
DyLight 488
NBP2-75775G
DyLight 550
NBP2-75775R
DyLight 594
NBP2-75775DL594
DyLight 650
NBP2-75775C
DyLight 680
NBP2-75775FR
DyLight 755
NBP2-75775IR
FITC
NBP2-75775F
HRP
NBP2-75775H
Janelia Fluor 525
NBP2-75775JF525
Janelia Fluor 549
NBP2-75775JF549
Janelia Fluor 646
NBP2-75775JF646
Janelia Fluor 669
NBP2-75775JF669
PE
NBP2-75775PE
PerCP
NBP2-75775PCP
Unconjugated
NBP2-75773-100ug
mFluor Violet 450 SE
NBP2-75775MFV450
mFluor Violet 500 SE
NBP2-75775MFV500
mFluor Violet 610 SE
NBP2-75775MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-75773-100ug
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UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75773).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human UCH-L1/PGP9.5 protein (Uniprot: P09936)
Localization
Cytoplasmic. Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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UCH-L1/PGP9.5
Antibody (rUCHL1/775) ...
NBP2-75775
UCH-L1/PGP9.5 Antibody (rUCHL1/775) - Azide and BSA Free
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, MA

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Bioinformatics

Gene Symbol UCHL1
Uniprot