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UCH-L1/PGP9.5 Antibody (rUCHL1/775) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA
Clone
rUCHL1/775
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75775AF350
Alexa Fluor 405
NBP2-75775AF405
Alexa Fluor 488
NBP2-75775AF488
Alexa Fluor 532
NBP2-75775AF532
Alexa Fluor 594
NBP2-75775AF594
Alexa Fluor 647
NBP2-75775AF647
Alexa Fluor 700
NBP2-75775AF700
Alexa Fluor 750
NBP2-75775AF750
Allophycocyanin
NBP2-75775APC
Biotin
NBP2-75775B
DyLight 350
NBP2-75775UV
DyLight 405
NBP2-75775V
DyLight 488
NBP2-75775G
DyLight 550
NBP2-75775R
DyLight 594
NBP2-75775DL594
DyLight 650
NBP2-75775C
DyLight 680
NBP2-75775FR
DyLight 755
NBP2-75775IR
FITC
NBP2-75775F
HRP
NBP2-75775H
Janelia Fluor 525
NBP2-75775JF525
Janelia Fluor 549
NBP2-75775JF549
Janelia Fluor 646
NBP2-75775JF646
Janelia Fluor 669
NBP2-75775JF669
PE
NBP2-75775PE
PerCP
NBP2-75775PCP
Unconjugated
NBP2-75773-100ug
mFluor Violet 450 SE
NBP2-75775MFV450
mFluor Violet 500 SE
NBP2-75775MFV500
mFluor Violet 610 SE
NBP2-75775MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-75773-100ug
Azide and BSA Free
NBP2-75775
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UCH-L1/PGP9.5 Antibody (rUCHL1/775) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human UCH-L1/PGP9.5 protein (Uniprot: P09936)
Localization
Cytoplasmic. Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for UCH-L1/PGP9.5 Antibody (rUCHL1/775) [CoraFluor™ 1]

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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UCH-L1/PGP9.5
Antibody (rUCHL1/775) ...
NBP2-75775CL1
UCH-L1/PGP9.5 Antibody (rUCHL1/775) [CoraFluor™ 1]
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, MA

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Bioinformatics

Gene Symbol UCHL1