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TSLP Antibody - BSA Free

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Biological Strategies: Western Blot: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/Nga mice. Lysates were immunoblotted with ...read more
Immunohistochemistry: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/ Nga mice. Paraffin sections of skin biopsies from DNCB-induced AD-like ...read more
Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via ...read more
Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in ...read more
Expression of Tagged Proteins: Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. ...read more
Western Blot: TSLP Antibody - BSA Free [NB110-55234] - Validation in Mouse A-20 Cell Line. Loading: 15 ug of lysates per lane.Antibodies: TSLP (A: 0.5 ug/mL, B: 1 ug/mL, C: 2 ug/mL), 1h incubation at RT in 5% ...read more
Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with ...read more
Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 ug/ml. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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TSLP Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to 19 amino acids near the center of human TSLP. The immunogen is located within the amino acids 40-90 of TSLP. Amino Acid Sequence: SGTKSTEFNNTVSCSNRPH
Specificity
Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). NB110-55234 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSLP
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.25-4 ug/ml
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TSLP Peptide (NB110-55234PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-55234 in the following applications:

Publications
Read Publications using
NB110-55234 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for TSLP Antibody - BSA Free

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSLP Antibody (NB110-55234)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(1)
WB
(5)
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Filter By Species
Human
(2)
Mouse
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-55234 Applications Species
Kanninen T, Tao L, Romero R et al. Thymic stromal lymphopoietin participates in the host response to intra-amniotic inflammation leading to preterm labor and birth Human immunology 2023-07-06 [PMID: 37422429] (WB, Human) WB Human
Chen C, Dai CY, Han F Et al. Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis International journal of ophthalmology 2021-10-18 [PMID: 34667722] (WB, Mouse) WB Mouse
Kim YJ, Choi MJ, Bak DH et al. Topical administration of EGF suppresses immune response and protects skin barrier in DNCB-induced atopic dermatitis in NC/Nga mice Sci Rep 2018-08-09 [PMID: 30093649] (WB, Mouse) WB Mouse
Buchheit KM, Cahill KN, Katz HR et al. Thymic stromal lymphopoietin controls prostaglandin D2 generation in patients with aspirin-exacerbated respiratory disease. J. Allergy Clin. Immunol. 2015-12-12 [PMID: 26691435]

Details:
TSLP antibody was used for WB analysis of lysates from nasal polyp samples of patients with aspirin-exacerbated respiratory disease/AERD (Fig 4; Fig E3). The antibody was tested for specificity using its corresponding blocking peptide NB110-55234PEP and was also compared with anti-TSLP antibody (ABT330 from Millipore; Fig E2).
Sziksz E, Molnar K, Lippai R et al. Peroxisome proliferator-activated receptor-y and thymic stromal lymphopoietin are involved in the pathophysiology of childhood coeliac disease. Virchows Arch. 2014-09-04 [PMID: 25187315] (IHC-P, Human) IHC-P Human
Han Sang-Chul, Kang Gyeoung-Jin, Ko Yeong-Jong et al. External Application of Fermented Olive Flounder (Paralichthys olivaceus) Oil Alleviates Inflammatory Responses in 2,4-Dinitrochlorobenzeneinduced Atopic Dermatitis Mouse Model. Toxicological Research 2012-01-01 [PMID: 24278605] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse
Han Sang-Chul, Kang Gyeoung-Jin, Ko Yeong-Jong et al. Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via geneRation of CD4+CD25+Foxp3+ T cells. Bmc Immunology 2012-01-01 [PMID: 22873180] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse

Review for TSLP Antibody (NB110-55234) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-55234:
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WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TSLP NB110-55234
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/01/2017
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Summary

ApplicationWestern Blot
Sample TestedNasal tissue
SpeciesHuman

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ICC/IF Video Protocol

FAQs for TSLP Antibody (NB110-55234) (0)

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Recent Reviews

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Verified Customer
07/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TSLP
Entrez
Uniprot