TSG101 Antibody

Tumor susceptibility 101 protein (human TSG101 theoretical molecular weight 44kDa) is the mammalian homologue of the yeast protein Vps23 which plays a role in endosomal and multivesicular body trafficking. TSG101 forms part of the endosomal sorting complexes required for transport complex 1 (ESCRT-I), a cytosolic multiprotein complex consisting additionally of Vps28, Vps37 (A-D) and Mvb12 (A, B) or UBAP1 (1). TSG101 contains multiple domains including the amino terminal ubiquitin e2 variant (UEV) domain, a proline-rich (RRR) domain, a coiled coil (CC) domain, and a carboxy terminal alpha-helical/steadiness box (SB) domain (2). As part of the ESCRT-I complex, TSG101 interacts through its UEV domain with ubiquitinated membrane proteins and members of the ESCRT-0 complex. The UEV domain plays a critical role for various TSG101 functions such as protein sorting into multivesicular bodies and late endosomes, and in the process of viral budding. For example, TSG101 interacts with the intermediate-conductance, Ca2+ -activated K+ channel (KCa3.1), facilitating its targeting to the lysosome for degradation (3). Additionally, TSG101 has been implicated in the turnover of connexins such as connexins 43 and 45 (4). TSG101 plays a role in other cellular functions including transcriptional regulation, cytokinesis and cell growth (2).

Upon its initial discovery, TSG101 was recognized as a tumor suppressor protein due to the identification of deletions within the TSG101 gene in human breast carcinomas. However, re-examination of the initial findings argued against this function and supported that TSG101 promotes tumorigenesis (2). In agreement with this role, TSG101 expression is upregulated in several types of cancer including breast, ovarian, and colorectal carcinoma.

References

1. Schmidt, O., & Teis, D. (2012). The ESCRT machinery. Current Biology. https://doi.org/10.1016/j.cub.2012.01.028

2. Jiang, Y., Ou, Y., & Cheng, X. (2013). Role of TSG101 in cancer. Frontiers in Bioscience. https://doi.org/10.2741/4099

3. Balut, C. M., Gao, Y., Murray, S. A., Thibodeau, P. H., & Devor, D. C. (2010). ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes. American Journal of Physiology - Cell Physiology. https://doi.org/10.1152/ajpcell.00120.2010

4. Su, V., & Lau, A. F. (2014). Connexins: Mechanisms regulating protein levels and intercellular communication. FEBS Letters. https://doi.org/10.1016/j.febslet.2014.01.013

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, WB, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP1-80659	Applications	Species
Jiao W, Hao J, Liu JM et al. Mesenchymal stem cells-derived extracellular vesicle-incorporated H19 attenuates cardiac remodeling in rats with heart failure The Kaohsiung journal of medical sciences 2023-10-27 [PMID: 37885317] (Western Blot, Rat)	Western Blot	Rat
Kim EJ, Kim JY, Kim SO et al. The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2 Cell reports 2022-11-22 [PMID: 36417870] (WB, Human)	WB	Human
Hua Y, Dai C, He Q Et al. Autoantibody panel on small extracellular vesicles for the early detection of lung cancer Clin Immunol 2022-11-10 [PMID: 36356847] (WB, Human) Details: Citation using the PE version of this antibody.	WB	Human
Carotenuto P, Romano A, Barbato A et al. Targeting the MITF/APAF-1 axis as salvage therapy for MAPK inhibitors in resistant melanoma Cell reports 2022-11-08 [PMID: 36351409] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Majewska A, Brodaczewska K, Filipiak-Duliban A et al. miRNA Pattern in Hypoxic Microenvironment of Kidney Cancer-Role of PTEN Biomolecules 2022-05-11 [PMID: 35625614] (Western Blot)	Western Blot
Zar� M, Campodonico J, Cosentino N et al. Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest International Journal of Molecular Sciences 2021-07-28 [PMID: 34360827] (Western Blot)	Western Blot
Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials 2021-04-22 [PMID: 33890421]
Pospichalova V, Svoboda J, Dave Z et al. Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer. J Extracell Vesicles 2015-01-01 [PMID: 25833224] (WB)	WB
Gross JC, Chaudhary V, Bartscherer K, Boutros M. Active Wnt proteins are secreted on exosomes. Nat Cell Biol 2012-10-01 [PMID: 22983114]
Pauwels MJ, Xie J, Ceroi A et al. Choroid plexus-derived extracellular vesicles exhibit brain targeting characteristics Biomaterials 2022-11-01 [PMID: 36302306] (WB, Mouse)	WB	Mouse
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Rat	01/17/2018	View Summary Application Western Blot Sample Tested Rat astrocytes Species Rat Lot B103539
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	12/08/2017	View Summary Application Western Blot Sample Tested Primary mouse hepatocytes Species Mouse Lot B103539 Comments Comments mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

Array NBP1-80659

• TSG101 Antibodies
• TSG101 ELISA Kits
• TSG101 Proteins