TSG101 Antibodies
We offer TSG101 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each TSG101 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TSG101 Antibodies can be used in a variety of model species: Hamster, Human, Mouse, Primate, Rat. Use the list below to choose the TSG101 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TSG101 polyclonal antibodies and browse our TSG101 monoclonal antibody catalog.
Alternate Names for TSG101 Antibodies
anti-TSG101 antibody, anti-ESCRT-I complex subunit TSG101 antibody, anti-TSG10 antibody, anti-tumor susceptibility gene 10 antibody, anti-tumor susceptibility gene 101 antibody, anti-tumor susceptibility gene 101 protein antibody, anti-tumor susceptibility protein antibody, anti-VPS23 antibody
13 Results for "tsg101" in Primary Antibodies
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0035.jpg "Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Immunohistochemistry-Paraffin-NB200-112-img0026.jpg)
![Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] (NB200-112) detects TSG101 protein by flow cytometry analysis.Sample: THP-1 cell.Black: Unlabelled sample was used as a control.Red: TSG101 antibody [4A10] (NB200-112).Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Flow-Cytometry-NB200-112-img0027.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0009.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0018.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0020.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0033.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. Western blot analysis of exosomes revealed differential expression of TSG101 between HCT116-R exosomes and HCT116-P exosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53063-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0034.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Immunohistochemistry-Paraffin-NB200-112-img0024.jpg)
![ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-ELISA-NB200-112-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Knockdown-Validated-NB200-112-img0014.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of pECs-EVs from different women in secretory & proliferative phase of cycle. Representative TEM images of pECs-EVs from secretory (A,B) & proliferative phase (C,D). Scale bars: 50 nm (A,C); 100 nm (B,D). Representative nanoparticle tracking analysis plots of pECs-EVs from secretory (E) & proliferative phase (F). Western blot showing the presence of different canonical EV markers (CD63, CD9, Alix & TSG101) in secretory & proliferative phase pECs-EVs (n = 4) (G). Negative EV markers used were Calnexin (ER), beta -tubulin & the cytosolic form of DCXR (H). The same markers were evaluated in pECs protein extract as controls for EV enrichment of these markers. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-31020241529330.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. (A) Representative TEM images of exosomes at 22000x magnification (Left) HCT116-R exosomes & (Right) HCT116-P exosomes. (B) Enlarged planar view of a cup-shaped structure for single exosome in our samples at 87000x magnification. (C) Western blot analysis of exosomes revealed differential expression of TSG101, CD9, & CD63 between HCT116-R exosomes & HCT116-P exosomes. (D) Average size distribution & zeta potential of exosomes measured in Zetasizer. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31712601), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-310202415392526.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - EPS15 depletion affects late endosomal maturation.(A) Co-depletion of SPOPL an EPS15 has an additive effect on influenza A virus infection. (B) EPS15 depletion stabilizes ESCRT components HRS, STAM & TSG101. (C) EPS15 depletion affects LDL uptake in cells resulting in an accumulation of LDL in enlarged vacuoles (upper panel). Late endosomes, visualized by life-cell microscopy of GFP-RAB7, are enlarged in cells depleted of EPS15 (lower panel).DOI://dx.doi.org/10.7554/eLife.13841.016 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-310202415394543.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained by TSG101 antibody [4A10] (NB200-112) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-910202420131256.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-910202420104410.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-91020242041227.jpg)
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP1-80659] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80659-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0015.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0008.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human prostate shows moderate cytoplasmic positivity in glandular and in smooth muscle cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0012.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0013.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0014.jpg)
![Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Western-Blot-NBP2-67884-img0010.jpg "Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0007.jpg)
![Flow Cytometry: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Flow-Cytometry-NBP2-67884-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 with anti-TSG101 antibody [JJ0900] at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2/3: TSG101 knockdown Hela whole cell lysate (10 ug). NBP2-67884 was shown to specifically react with TSG101 in wild-type Hela cells. Weakened bands were observed when TSG101 knockdown samples were tested. Wild-type and TSG101 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Knockdown-Validated-NBP2-67884-img0012.jpg)
![Simple Western: TSG101 Antibody (JJ0900) [NBP2-67884] - Simple Western Lane view shows K562 whole cell lysate (WCL). A specific band was detected for TSG101 antibody (NBP2-67884) at approximately 53 kDa (as indicated) using 1:50 ug/mL of TSG101 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Simple-Western-NBP2-67884-img0013.jpg)
![Western Blot: TSG101 Antibody [NBP2-94822] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 40s.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-94822-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-94822] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 40s.")
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Rat kidney using TSG101/VPS23 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0007.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Human colon carcinoma using TSG101/VPS23 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Mouse kidney using TSG101/VPS23 Rabbit pAb t dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Rat kidney using TSG101/VPS23 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0006.jpg)
![Immunoprecipitation: TSG101 Antibody [NBP2-94822] - Analysis of 200ug extracts of HeLa cells using 3ug TSG101/VPS23 antibody. Western blot was performed from the immunoprecipitate using TSG101/VPS23 antibody (A1692) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunoprecipitation-NBP2-94822-img0008.jpg)
![Western Blot: TSG101 Antibody [NBP2-94900] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-94900-img0004.jpg "Western Blot: TSG101 Antibody [NBP2-94900] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-94900] - Analysis of L929 cells using TSG101 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94900-img0001.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94900] - Analysis of 150ug extracts of HeLa cells using TSG101 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94900-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94900] - Paraffin-embedded Mouse kidney using TSG101 .](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94900-img0002.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-RB-Western-Blot-NBP3-13656-img0001.jpg "Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-9102024202190.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-91020242001026.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-1610202419414543.jpg)
![Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474218..jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from PC-12 cells using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime:10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474338..jpg)