| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77452SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80659-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94822-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94900-0.02ml | |||
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP1-80659] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80659-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0015.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0008.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human prostate shows moderate cytoplasmic positivity in glandular and in smooth muscle cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0012.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0013.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0014.jpg)
![Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Western-Blot-NBP2-67884-img0010.jpg "Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0007.jpg)
![Flow Cytometry: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Flow-Cytometry-NBP2-67884-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 with anti-TSG101 antibody [JJ0900] at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2/3: TSG101 knockdown Hela whole cell lysate (10 ug). NBP2-67884 was shown to specifically react with TSG101 in wild-type Hela cells. Weakened bands were observed when TSG101 knockdown samples were tested. Wild-type and TSG101 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Knockdown-Validated-NBP2-67884-img0012.jpg)
![Simple Western: TSG101 Antibody (JJ0900) [NBP2-67884] - Simple Western Lane view shows K562 whole cell lysate (WCL). A specific band was detected for TSG101 antibody (NBP2-67884) at approximately 53 kDa (as indicated) using 1:50 ug/mL of TSG101 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Simple-Western-NBP2-67884-img0013.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-RB-Western-Blot-NBP3-13656-img0001.jpg "Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-9102024202190.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-91020242001026.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-1610202419414543.jpg)
![Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516321425.jpg "Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from K-562 cells using TSG101 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [TSG101] - Western blot analysis of lysates from wild type (WT) and TSG101 knockdown (KD) SH-SY5Y cells using TSG101 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-52202516283277.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251638068.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [TSG101] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94822_rabbit-polyclonal-tsg101-antibody-5220251626465.jpg)
![Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519294535.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of extracts from normal (control) and TSG101 knockdown (KD) SH-SY5Y(KD) cells, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Western blot analysis of K-562, using TSG101 Rabbit pAb (A2216) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519302972.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of Jurkat cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-52202519313476.jpg)
![Immunocytochemistry/ Immunofluorescence: TSG101 Antibody - Azide and BSA Free [NBP2-94900] - Immunofluorescence analysis of PC-12 cells using TSG101 Rabbit pAb (A2216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94900_rabbit-polyclonal-tsg101-antibody-522025193131.jpg)
![SDS-Page: Recombinant Human TSG101 Protein [H00007251-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-TSG101-Protein-SDS-Page-H00007251-P01-img0002.jpg "SDS-Page: Recombinant Human TSG101 Protein [H00007251-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474218..jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from PC-12 cells using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime:10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474338..jpg)
![Western Blot:[NBP3-30702]-Detection of human and mouse TSG101 by western blot. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-TSG101 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30702_rabbit-tsg101-pab-23120251225614.jpg "Western Blot:[NBP3-30702]-Detection of human and mouse TSG101 by western blot. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-TSG101 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![SDS-Page: Recombinant Human TSG101 Protein [NBP1-44489] - TSG101, 20.7 kDa (181aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/TSG101-Protein-SDS-Page-NBP1-44489-img0001.jpg "SDS-Page: Recombinant Human TSG101 Protein [NBP1-44489] - TSG101, 20.7 kDa (181aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")
![ELISA: Human TSG101 ELISA Kit (Colorimetric) [NBP3-38762] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38762_human-tsg101-elisa-kit-colorimetric-2110202415384054.png "ELISA: Human TSG101 ELISA Kit (Colorimetric) [NBP3-38762] - Standard Curve Reference")