Western Blot: TRPM2 Antibody [NB500-242] - Samples: Jurkat Membrane Prep (50, 15, 5 ug). Antibody: Affinity purified rabbit anti-TRPM2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure ...read more
Immunoprecipitation: TRPM2 Antibody [NB500-242] - Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody NB500-242 used for ...read more
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human Transient Receptor Potential Cation Channel, Subfamily M, Member 2 using the numbering given in entry NP_003298.1 (GeneID 7226).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPM2
Purity
Immunogen affinity purified
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Estrogen-responsive element-associated gene 1 protein
KNP3LTrpC-2
Long transient receptor potential channel 2
LTrpC2
LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
NUDT9H
NUDT9L1
transient receptor potential cation channel, subfamily M, member 2
Transient receptor potential channel 7
TrpC7
Background
TRPM2 is a Ca(2+)-permeable channel that is activated by oxidative stress and confers susceptibility to cell death.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TRPM2 Antibody (NB500-242). (Showing 1 - 1 of 1 FAQs).
I have tested this antibody and would like to share the results from my first preliminary experiment. This Ab has been tested in ICC using a human cell line. Blocking buffer contains either goat or donkey serum. Dilutions: 1:50 and 1: 100 have been tested. Secondary Ab is goat anti rabbit FITC (1:400). Control test: No signal is detected when TRPC4 is omitted. Results: a bright staining around the entire cell structure including the cell membrane has been detected in every single cell. Questions: (1) Is an absorption test absolutely required before I can confidently say the staining is specific? If yes, what's your suggested protocol? (2) How can confirm the staining is in the cell membrane?
TRPM2 is expected to be expressed on the cell membrane. It sounds to me like you are seeing membrane-bound staining so it appears to be working just fine. As far as whether or not you need to do an absorption test and whether or not you believe that the staining is truly in the cell membrane, you may want to consult your mentor as they should be able to interpret data on these proteins better than I can.
Winter is coming, and TRPM8 welcomes the cold! TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds. While TRPM8 is best known for its location in peripheral nerve endings, it has functio... Read full blog post.
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