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TRPM2 Antibody - BSA Free

Images

 
Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody [NB110-82364] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.5 mg/ml

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TRPM2 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse TRPM2 protein (within residues 1200-1300). [Swiss-Prot# Q91YD4]
Localization
Cell membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 2 ug/ml
Application Notes
This TRP7 antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~172 kDa. In ICC/IF punctate membrane staining was observed in Neuro2A cells.
Theoretical MW
172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Brain Membrane Tissue Lysate (Adult Membrane Normal)
Control Peptide
TRPM2 Peptide (NB110-82364PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPM2 Antibody - BSA Free

  • EC 3.6.1.13
  • EREG1MGC133383
  • Estrogen-responsive element-associated gene 1 protein
  • KNP3LTrpC-2
  • Long transient receptor potential channel 2
  • LTrpC2
  • LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
  • NUDT9H
  • NUDT9L1
  • transient receptor potential cation channel, subfamily M, member 2
  • Transient receptor potential channel 7
  • TrpC7

Background

TRP7 (transient receptor protein 7 or TRPC7) is the seventh identified member of mammalian TRPC channel family which includes nonselective cation channels that mediate changes in plasma membrane cation permeability induced by stimulation of PLC (phospholipase C, the cellular integrator that receives input from GPCRs/ RTK). Major cellular function of TRPC channels is to regulate Ca2+ influx across plasma membrane induced by stimulation of these receptors by hormones, neurotransmitters, and growth factors. Localized in the cell membrane and nuclear envelope as multi-pass membrane protein, TRPC7 interacts with with MX1, RNF24, PRKG1 etc. and PRKG1 mediated TRPC7 phosphorylation at Thr-15 site negatively regulates TRPC7's activity. TRP7 has been suggested to form a receptor-activated non-selective calcium permeant cation channel and is operated by a phosphatidylinositol second messenger system activated by RTKs or GPCRs. TRP7 is activated by DAG (diacylglycerol) and also upon intracellular calcium store depletion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for TRPM2 Antibody (NB110-82364): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPM2 Antibody (NB110-82364) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Array NB110-82364

Research Areas for TRPM2 Antibody (NB110-82364)

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TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

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Bioinformatics

Gene Symbol TRPM2