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TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

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Western Blot: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2.
Western Blot: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2.
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human breast tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human endometrium. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74577AF350
Alexa Fluor 405
NBP2-74577AF405
Alexa Fluor 488
NBP2-74577AF488
Alexa Fluor 532
NBP2-74577AF532
Alexa Fluor 594
NBP2-74577AF594
Alexa Fluor 647
NBP2-74577AF647
Alexa Fluor 700
NBP2-74577AF700
Alexa Fluor 750
NBP2-74577AF750
Biotin
NBP2-74577B
CoraFluor 1
NBP2-74577CL1
DyLight 350
NBP2-74577UV
DyLight 405
NBP2-74577V
DyLight 488
NBP2-74577G
DyLight 550
NBP2-74577R
DyLight 594
NBP2-74577DL594
DyLight 650
NBP2-74577C
DyLight 680
NBP2-74577FR
DyLight 755
NBP2-74577IR
FITC
NBP2-74577F
HRP
NBP2-74577H
Janelia Fluor 525
NBP2-74577JF525
Janelia Fluor 549
NBP2-74577JF549
Janelia Fluor 646
NBP2-74577JF646
Janelia Fluor 669
NBP2-74577JF669
Unconjugated
NBP2-46228
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46228
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TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-241 of human NR2C2(NP_003289) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NR2C2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
67.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

  • hTAK1
  • NR2C2
  • Nuclear hormone receptor TR4
  • nuclear receptor subfamily 2 group C member 2
  • nuclear receptor subfamily 2, group C, member 2
  • Orphan nuclear receptor TAK1
  • Orphan nuclear receptor TR4
  • TAK1
  • TAK1TR4TR2R1
  • Testicular receptor 4
  • TR4 nuclear hormone receptor
  • TR4

Background

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NR2C2