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TR4/NR2C2 Antibody (2A5)

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Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of NR2C2 over-expressed 293 cell line, cotransfected with NR2C2 Validated Chimera RNAi ( Cat # H00007182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR2C2 monoclonal antibody (M01), clone 2A5 (Cat # H00007182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of NR2C2 over-expressed 293 cell line, cotransfected with NR2C2 Validated Chimera RNAi ( Cat # H00007182-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of monoclonal antibody to NR2C2 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.
Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of NR2C2 expression in transfected 293T cell line by NR2C2 monoclonal antibody (M01), clone 2A5.Lane 1: NR2C2 transfected lysate(65.414 KDa).Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, KD
Clone
2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TR4/NR2C2 Antibody (2A5) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
NR2C2 (NP_003289, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KIVTDQQTGQKIQIVTAVDASGSPKQQFILTSPDGAGTGKVILASPETSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKTDVQRPQVVEYCVVCGDKASGRHYGAVS
Localization
Nuclear
Specificity
NR2C2 - nuclear receptor subfamily 2, group C, member 2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NR2C2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TR4/NR2C2 Antibody (2A5)

  • hTAK1
  • NR2C2
  • Nuclear hormone receptor TR4
  • nuclear receptor subfamily 2 group C member 2
  • nuclear receptor subfamily 2, group C, member 2
  • Orphan nuclear receptor TAK1
  • Orphan nuclear receptor TR4
  • TAK1
  • TAK1TR4TR2R1
  • Testicular receptor 4
  • TR4 nuclear hormone receptor
  • TR4

Background

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NR2C2
Entrez
OMIM
Uniprot