| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human TopBP1 Met88-Asp194 Accession # Q92547 |
| Specificity | Detects human TopBP1 in direct ELISAs. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | TOPBP1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
TopBP1 (DNA topoisomerase 2-binding protein 1) is a 175-180 kDa member of a critical DNA replication repair complex. It is widely expressed, and appears to serve as an intermediary in DNA replication. When a replication fork is stalled during S phase, DNA continues to unwind ahead of the stall. This becomes associated with RPA, which is essential for ATR and subsequent Chk1 activation. TopBP1 is key to ATR activation, and appears to first bind to DNA:pol-alpha , followed by a 9-1-1 complex and Rad17 interaction that leads to ATR stimulation. Human TopBP1 is 1522 amino acids (aa) in length. It contains eight BRCT domains (aa 101-1486) that interact with distinct binding partners. BRCT1 and 2 binds to Rad9 (aa 101-294), while BRCT 6 and 7 are involved in ATR activation. There are 12 utilized phosphorylation sites and two nuclear localization signals. Three isoform variants have been described. One contains an alternative start site at Met88, a second shows an 18 aa substitution for aa 1198-1522, and a third possesses a six aa substitution for aa 1292-1522. Over aa 88-194, human TopBP1 shares 88% aa identity with mouse TopBP1.
Secondary Antibodies |
Isotype Controls |
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