I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

TNF-alpha Antibody [Unconjugated] (AB-410-NA): Novus Biologicals

- COVID-19 Research Tools
  
  Antibodies
  Primary Antibodies
  Conjugated Antibodies
  
  Sample Size Antibodies
  
  KO Validated Antibodies
  
  Recombinant Antibodies
  
  Antibody Sampler Packs
  
  Antibody Pairs
  
  Secondary Antibodies
  
  Isotype Control Antibodies
  
  Proteins and Peptides
  Peptides & Proteins
  
  Blocking Peptides
  
  Biologically Active Proteins
  
  Lysates and Cell Lines
  Lysates
  
  Stem Cell Lines
- Kits
  Antibody Labeling Kits
  
  ChIP Kits
  
  ELISA Kits
  
  Flow Cytometry Kits
  
  Activators and Inhibitors
  Inhibitors
  
  Activators
  
  Support Products
  Exosome Isolation
  
  Flow Cytometry Beads
  
  IHC Tissue Slides
  
  IHC Microarrays
  
  Western Blot Gels & Buffers
  
  Western Blot Membranes
  
  All Support Products
  
  Bulk and Custom
Products
Our Protocols
- Resources
  Protocols
  
  Video Protocols
  
  Troubleshooting Guides
  
  Posters and Literature
  
  Handbooks and Guides
  
  Calculators
  
  FAQs
  
  Blogs
  
  Diseases
  
  Whitepapers
  
  Antibody Concierge
  
  Webinars
- Tools
  Spectra Viewer
  
  Flow Cytometry Panel Builder
  
  Application
  ELISA
  
  Flow Cytometry
  
  Mass Cytometry (CyTOF)
  
  ICC
  
  IHC
  
  Western Blotting
  
  Interviews
  Faculty
  
  Postdoc
Resources
View Pathways
- By Research Area
  Apoptosis
  
  Autophagy
  
  Cancer
  
  Cell Biology
  
  Cellular Markers
  
  Epigenetics
  
  Epitope Tags
  
  Exosomes Research
  
  Hypoxia
  
  Immunology
  
  Microbiology
  
  Neuroscience
  
  Stem Cells
- By Pathway
  AKT
  
  Apoptosis
  
  Autophagy
  
  ERK1/2/5 Pathway
  
  Hedgehog
  
  Hippo
  
  JNK Pathway
  
  mTOR
  
  Necroptosis
  
  NFKB Signaling
  
  p38 Signaling
  
  TGF Beta
  
  TLR
Research Areas
Get Rewards
- Customer Care
  100% Guarantee
  
  Risk Free Testing
  
  Novus Rewards Program
  
  Publication Rewards Program
  
  New Lab Discount
  
  Scholarship
  
  Travel Grants
  
  Promotions
Customer Care
Novus Careers
- About us
  Careers
  
  Collaborations
  
  Events
  
  Novus Story
  
  Our Team
  
  Philanthropy
  
  Press Releases
  
  Why choose Novus
About Us
- Contact Us
  Customer Service
  
  Distributors
  
  Technical Support
Contact

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, B/N
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Immunogen	E. coli-derived recombinant mouse TNF-alpha Leu80-Leu235 Accession # P06804
Specificity	Detects mouse TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with human TNF‑ alpha is observed, and less than 5% cross-reactivity with recombinant bovine TNF‑ alpha , recombinant canine TNF‑ alpha , recombinant equine TNF‑ alpha , recombinant feline TNF‑ alpha , and recombinant porcine TNF‑ alpha is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TNF
Purity Statement	Protein A or G purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 1 mg/mL in sterile PBS.

Publications using AB-410-NA	Applications	Species
M Sakai, Z Yu, M Taniguchi, R Picotin, N Oyama, D Stellwagen, C Ono, Y Kikuchi, K Matsui, M Nakanishi, H Yoshii, T Furuyashik, T Abe, H Tomita N-Acetylcysteine Suppresses Microglial Inflammation and Induces Mortality Dose-Dependently via Tumor Necrosis Factor-alpha Signaling International Journal of Molecular Sciences, 2023-02-14;24(4):. 2023-02-14 [PMID: 36835209] (IHC, Mouse, Transgenic Mouse)	IHC	Mouse, Transgenic Mouse
X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration Disease Models & Mechanisms, 2021-12-24;14(12):. 2021-12-24 [PMID: 34821368] (Neutralization, Mouse)	Neutralization	Mouse
K Sato, A Tobo, C Mogi, M Tobo, N Yamane, M Tosaka, H Tomura, DS Im, F Okajima The protective role of proton-sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model Sci Rep, 2020-10-14;10(1):17193. 2020-10-14 [PMID: 33057165] (Western Blot, Mouse)	Western Blot	Mouse
Xu, C, Wang, L Et al. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms. J Am Soc Nephrol 5/1/2020 [PMID: 32253273]
Y Ono, Y Maejima, M Saito, K Sakamoto, S Horita, K Shimomura, S Inoue, J Kotani TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice Sci Rep, 2020-01-20;10(1):694. 2020-01-20 [PMID: 31959927] (Cell Culture, Mouse)	Cell Culture	Mouse
S Mahmoudi, E Mancini, L Xu, A Moore, F Jahanbani, K Hebestreit, R Srinivasan, X Li, K Devarajan, L Prélot, CE Ang, Y Shibuya, BA Benayoun, ALS Chang, M Wernig, J Wysocka, MT Longaker, MP Snyder, A Brunet Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing Nature, 2019-10-23;574(7779):553-558. 2019-10-23 [PMID: 31645721] (Cell Culture, Mouse)	Cell Culture	Mouse
Y Ono, K Sakamoto Lipopolysaccharide inhibits myogenic differentiation of C2C12 myoblasts through the Toll-like receptor 4-nuclear factor-?B signaling pathway and myoblast-derived tumor necrosis factor-? PLoS ONE, 2017-07-24;12(7):e0182040. 2017-07-24 [PMID: 28742154] (Neutralization, Mouse)	Neutralization	Mouse
Chang Liu, Angana G. Rajapakse, Erwin Riedo, Benoit Fellay, Marie-Claire Bernhard, Jean-Pierre Montani et al. Targeting arginase-II protects mice from high-fat-diet-induced hepatic steatosis through suppression of macrophage inflammation Scientific Reports 2016-02-05 [PMID: 26846206]
Zhao Y, Xiao X, Frank S, Lin H, Xia Y Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta. Am J Physiol Endocrinol Metab, 2014-06-03;307(2):E186-98. 2014-06-03 [PMID: 24895283] (Neutralization, Mouse)	Neutralization	Mouse
Devavani Chatterjea, Luisa Paredes, Tijana Martinov, Evelyn Balsells, Juliann Allen, Akilah Sykes et al. TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation F1000Research 2013-09-27 [PMID: 24555087]
Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination Nature Neuroscience 2013-09-01 [PMID: 23872599]
Julie Norseen, Tetsuya Hosooka, Ann Hammarstedt, Mark M. Yore, Shashi Kant, Pratik Aryal et al. Retinol-Binding Protein 4 Inhibits Insulin Signaling in Adipocytes by Inducing Proinflammatory Cytokines in Macrophages through a c-Jun N-Terminal Kinase- and Toll-Like Receptor 4-Dependent and Retinol-Independent Mechanism Molecular and Cellular Biology 2012-05-01 [PMID: 22431523]
William D. Stuart, Rishikesh M. Kulkarni, Jerilyn K. Gray, Juozas Vasiliauskas, Mike A. Leonis, Susan E. Waltz The Ron Receptor Regulates Kupffer Cell-Dependent Cytokine Production and Hepatocyte Survival Following Endotoxin Exposure in Mice Hepatology 2011-05-01 [PMID: 21520175]
Toru Nakazawa, Maki Kayama, Morin Ryu, Hiroshi Kunikata, Ryou Watanabe, Masayuki Yasuda et al. Tumor necrosis factor-alpha mediates photoreceptor death in a rodent model of retinal detachment Investigative Opthalmology & Visual Science 2011-03-16 [PMID: 21402953]
Hashiramoto A, Yamane T, Tsumiyama K, Yoshida K, Komai K, Yamada H, Yamazaki F, Doi M, Okamura H, Shiozawa S Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha. J. Immunol., 2009-12-30;184(3):1560-5. 2009-12-30 [PMID: 20042581] (Neutralization, Mouse)	Neutralization	Mouse
Quintao NL, Balz D, Santos AR, Campos MM, Calixto JB Long-lasting neuropathic pain induced by brachial plexus injury in mice: Role triggered by the pro-inflammatory cytokine, tumour necrosis factor alpha. Neuropharmacology, 2005-12-28;50(5):614-20. 2005-12-28 [PMID: 16386767] (Neutralization, Mouse)	Neutralization	Mouse
Show All 16 Publications. Collapse Publications.

TNF-alpha Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

TNF-alpha Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for TNF-alpha Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for TNF-alpha Antibody (AB-410-NA)(16)

Reviews for TNF-alpha Antibody (AB-410-NA) (0)

Product General Protocols

Video Protocols

FAQs for TNF-alpha Antibody (AB-410-NA). (Showing 1 - 4 of 4 FAQs).

Secondary Antibodies

Isotype Controls

Additional TNF-alpha Products

Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics

Gene Symbol	TNF
Uniprot	P06804()