TNF-alpha Antibodies
Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine secreted by monocytes/macrophages, T cells, and natural killer (NK) cells. TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa as well as a 157 aa, 17 kDa soluble protein (sTNF-alpha). We offer TNF-alpha antibodies featured in 330+ publications, including multiple 5-star customer reviewed antibodies. Browse our inventory of recombinant monoclonal, monoclonal, polyclonal, and TNF-alpha-conjugated antibodies. Validated research applications include Blocking/Neutralizing, CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Western Blot, and more. Our TNF-alpha antibodies are reactive in a range of species such as Human, Mouse, Rat, Canine, Feline, Guinea Porcine, Primate, Rabbit, and Zebrafish. Each antibody fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it. Choose the anti-TNF-alpha antibody best suited for your research needs by viewing the technical details, images, references, and related products.
Alternate Names for TNF-alpha Antibodies
anti-TNF-alpha antibody, anti-TNF antibody, anti-APC1 protein antibody, anti-Cachectin antibody, anti-DIF antibody, anti-TNF, monocyte-derived antibody, anti-TNF-a antibody, anti-TNF-alphacachectin antibody, anti-TNFATNF, macrophage-derived antibody, anti-TNFSF2TNF superfamily, member 2 antibody, anti-tumor necrosis factor (TNF superfamily, member 2) antibody, anti-tumor necrosis factor alpha antibody, anti-tumor necrosis factor antibody, anti-Tumor necrosis factor ligand superfamily member 2 antibody, anti-tumor necrosis factor-alpha antibody
91 Results for "tnf-alpha" in Primary Antibodies
![WNT-5A induces a proinflammatory transformation in mouse microglia. (A, B) iNOS, COX-2 and TNF alpha were detected by immunoblotting in lysates from mouse primary microglia after WNT-5A stimulation (ctrl, 300 ng/ml, 6 hours). (B’) shows TNF alpha levels in the supernatant of primary microglia under ctrl conditions and upon WNT-5A stimulation (ctrl, 300 ng/ml, 24 hours; n = 4). At least three experiments are summarized in the bar graphs. Data are normalized to ctrl. Error bars give s.e.m. (C) Microglial proliferation was assessed by an MTT assay monitoring mitochondrial activity, which is proportional to cell number [see Additional file 1: Figure S5]. Stimulation with WNT-5A (300 ng/ml, 24 hours) increased MTT, which was blocked by PTX (100 ng/ml, overnight) or the MEK1/2 (10 μM) inhibitor, SL327. (D). Experiments were done in triplicate and data from three independent experiments are shown. *, P < 0.01; ***, P < 0.001: Error bars show s.e.m.. (E) Cell tracker (red)-stained primary microglia were seeded on top of a collagen matrix in 35 mm glass bottom dishes. One day after ctrl or 300 ng/ml WNT-5A stimulation, invasion was observed by confocal microscopy and Z-stacking using a Zeiss LSM710 microscope and subsequent analysis with the Bitplane Imaris software. The size of the collagen cube shown is 1,000 (Z) x 1,400 (Y) x 1,400 (X) μm. Three invasion experiments in the absence and presence of the MEK1/2 inhibitor SL327 were quantified. Data are presented in a bar graph (F). *, P < 0.05; error bars show s.e.m.. (G) cDNA of ctrl stimulated (−) and WNT-5A stimulated (+) primary microglia was analyzed by QPCR for expression of inflammatory genes. At least three independent experiments are summarized. Gene expression is normalized to the housekeeping gene GAPDH and expressed as arbitrary units (2-delta ct). *, P < 0.05; **, P < 0.01. COX-2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; n, number; s.e.m., standard error of the mean. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af-410-na_mouse-tnf-alpha-affinity-purified-polyclonal-ab-41202410331929.jpg)
![Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0007.jpg "Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, P < 0.001 versus NS group; &P < 0.001 versus low-dose group. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/mi/2013/432623/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0008.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected diffused immunostaining of this protein in the tested tissue. Staining was primarily observed in the epithelial cells and some cells showed membrane positivity also.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0005.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected immunopositivity of this protein in the tested tissue. Staining was primarily observed in the epithelial cells while some staining was present in mucosa muscularis also.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0006.jpg)
![Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-75925-img0002.jpg "Western Blot: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human TNF-alpha Monoclonal Antibody (NBP2-75925) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TNF-alpha at approximately 18 and 21 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (2342C) - Low Endotoxin and Azide Free [NBP2-75925] - Analysis of a FFPE tissue section of the human intesting using 1:200 dilution of TNF-alpha antibody (NBP2-75925). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a moderate to strong cytoplasmic staining in crypt cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-75925-img0003.jpg)
![Neutralization: TNF-alpha Antibody (2342C) [NBP2-75925] - Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # NBP2-75925). The ND50 is typically 1-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-2342C-Neutralization-NBP2-75925-img0001.jpg)
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0012.jpg "Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Analysis using Azide/BSA FREE version of NB600-587. Membrane blocked in 1% BSA-TBS-T 30 min RT, 1:1000 dilution in 1% BSA-TBS-T 4C.")
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P<0.001, compared with the controls; #P<0.001 compared with the previous time point). Five rats in each group were used for western blot (n=5), sham, sham-operated control rats Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody - Azide Free [NB600-587] - LMP2 knockdown by shRNA reduced the expression of TNF-alpha and IL-1beta after MCAO. Immunofluorescence labeling revealed that both TNF-alpha and IL-1beta immunoreactivity was significantly upregulated in the ipsilateral ischemic hemisphere compared with sham-operated animals. In contrast, LMP2 knockdown by shRNA significantly reduced the expression of TNF-alpha and IL-1beta. Five rats in each group were used for immunofluorescent assays (n=5). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NB600-587-img0015.jpg)
![Immunohistochemistry: TNF-alpha Antibody - Azide Free [NB600-587] - Staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Immunohistochemistry-NB600-587-img0008.jpg)
![Western Blot: TNF-alpha Antibody - Azide Free [NB600-587] - Expression of TNF-alpha, IL-1beta and NF-kappaB in the brains of MCAO rats. Western blot confirmed that protein levels of TNF-alpha increased as early as 4 h after reperfusion, further increased at 24 h, peaked at 72 h and decreased at 7 days, respectively (*P<0.001, compared with the controls; #P<0.001 compared with the previous time point). Five rats in each group were used for western blot (n=5), sham, sham-operated control rats Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014586), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody---Azide-Free-Western-Blot-NB600-587-img0013.jpg)
![IL-10 receptors expressed on hippocampal neurons.(A) Expression of IL-10 receptor mRNAs in hippocampal neurons. The expression of the IL-10 receptor was identified using RT-PCR. The mRNAs of IL-10 receptor alpha and beta were expressed in the hippocampal neurons. IL-10 receptor alpha was expressed mainly in hippocampal neurons of DIV 7. (1, cultured hippocampal neurons at DIV 7; 2, cultured hippocampal neurons at DIV 15; 3, mixed glial culture at DIV 7; 4, mixed glial culture at DIV 15; 5, microglia) Quantification (DIV 15 neuron/ DIV 7 neuron): IL-10 receptor alpha , 0.61; IL-10 receptor beta , 1.06. (B) Expression of IL-10 receptor proteins in cultured hippocampal neurons. Similar to the expression of mRNA, the IL-10 receptor alpha protein was expressed mainly in neurons of DIV 7. Anti-IL-10 receptor alpha antibodies (0.5 μg/ml, Santa Cruz, sc-985) were used for western blotting [27]. Quantification (DIV 15 neuron/ DIV 7 neurons): IL-10 receptor alpha , 0.73.(C) Expression of IL-10 receptor proteins in the developing rat brains. The IL-10 receptor alpha proteins were expressed mainly in the developing brains of embryonic and early postnatal days (E18~P3). Quantification of IL-10 receptor alpha : E18, 0.30; P1, 0.27; P3, 1.0; P7, 0.22; P14, 0.20; P3W, 0.17; P6W, 0.15 (E, embryonic days; P, postnatal days).(D) Images of IL-10 receptor expressions in cultured hippocampal neurons. Hippocampal neurons of DIV 6 were stained with antibodies of IL-10 receptor alpha (5 μg/ml, Santa Cruz, sc-985) (red) and MAP2 (the neuronal marker, green) after treatment with 4% formaldehyde and then -20 °C methanol. Hippocampal neurons expressed IL-10 receptor proteins comparable to spinal neurons or cortical neurons.(E) The induction of synaptic formation by microglia was antagonized by the neutralizing antibody of IL-10 receptor alpha . When anti-mouse IL-10 receptor alpha antibody was applied to the co-culture of mouse microglia and mouse hippocampal neurons, the density of dendritic spines was significantly decreased compared with the control (without anti-IL-10 receptor antibody). Means±SEM. n=30 dendrites for no microglia without anti-IL-10 receptor antibody, 29 for the application of microglia only, 29 for no microglia with anti-IL-10 receptor antibody only, 29 for microglia with anti-IL-10 receptor antibody. ***p<0.001 by the Newman-Keuls multiple comparison test after application of one-way ANOVA, F=17.35, p<0.0001.(F) The induction of synaptic formation via microglia was not antagonized by the blocking antibody of TNF alpha . When anti-rat TNF alpha antibody was applied to the co-culture of rat microglia and rat hippocampal neurons, the density of dendritic spine was not decreased compared with control (without anti-TNF alpha antibody). Means±SEM. n=27 dendrites for no microglia without anti-TNF alpha antibody, 28 for the application of microglia only, 29 for no microglia with anti-TNF alpha receptor antibody only, 27 for microglia with anti-TNF alpha antibody. **p<0.01 and ***p<0.001 by the Newman-Keuls multiple comparison test after application of one-way ANOVA, F=9.104, p<0.0001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24278397), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab510_rat-tnf-alpha-mab-clone-45418-41202412511313.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-52B83-Immunohistochemistry-Paraffin-NB600-1422-img0001.jpg "Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.")