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Thymidine Kinase 1 Antibody (6F6)

Images

 
Western Blot: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Positive Western Blot detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, ...read more
Immunocytochemistry/ Immunofluorescence: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Staining of Hela with Thymidine Kinase 1 Antibody (6F6) at 1:40, counter-stained with DAPI. The cells were fixed in 4% ...read more
Flow Cytometry: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Overlay Peak curve showing Hela cells stained with Thymidine Kinase 1 Antibody (6F6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
6F6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Thymidine Kinase 1 Antibody (6F6) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human Thymidine Kinase 1 [UniProt P04183]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TK1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Thymidine Kinase 1 Antibody (6F6)

  • EC 2.7.1.21
  • thymidine kinase 1 soluble isoform
  • Thymidine Kinase 1
  • thymidine kinase 1, soluble
  • thymidine kinase, cytosolic
  • thymidine kinase-1
  • TK1
  • TK2

Background

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TK1