![Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Western-Blot-NBP1-52410-img0010.jpg "Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Po, Bv, Eq, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Immunogen | Peptide with sequence NRIPESGGDNSVFD-C, from the N Terminus of the Thrombospondin-1 protein sequence according to NP_003237.2. |
| Epitope | N-Terminus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | THBS1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC: NBP1-52410 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Thrombospondin-1 antibody validated for FLOW from a verified customer review.. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH 7.3) and 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Antigen Affinity-purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Deshea Harris |
Flow | Human | 11/23/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Thrombospondin-1 Antibody (NBP1-52410)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52410-img0008.jpg "Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane staining.")
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![Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with Spleenocytes. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-Flow-Cytometry-NBP1-52410-img0003.jpg "Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with Spleenocytes. Image submitted by a verified customer review.")
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