| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA(Det) |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 700 | FAB2411N-100 | |||
| Alexa Fluor 700 | FAB2411N-025 | |||
| Allophycocyanin | FAB2411A-100 | |||
| Allophycocyanin | FAB2411A-025 | |||
| Fluorescein | FAB2411F-100 | |||
| Fluorescein | FAB2411F-025 | |||
| Phycoerythrin | FAB2411P-100 | |||
| Phycoerythrin | FAB2411P-025 | |||
| Unconjugated | AF-241-NA | |||
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII Ile24-Asp159 Accession # P37173 |
| Specificity | Detects human TGF-beta RII in ELISAs and Western blots. In sandwich immunoassays, approximately 25% cross-reactivity with recombinant human (rh) TGF-beta RIIb is observed and less than 0.05% cross-reactivity with rhTGF-beta RI, rhTGF-beta RIII, recombinant mouse TGF-beta RII, and rhTGF-beta 1 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TGFBR2 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA Capture: Human TGF-beta RII Antibody (Catalog # AF-241-NA) ELISA Detection: Human TGF-beta RII Biotinylated Antibody (Catalog # BAF241) Standard: Recombinant Human TGF-beta RII (Catalog # 241-R2) |
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| Readout System | ||
| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Most cell types express three sizes of receptors for TGF-beta . These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.
The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .
![TGF-beta RII Antibody [Biotin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "TGF-beta RII Antibody [Biotin]")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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