TGF-beta 3 Antibody (20724) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF‑ beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Chicken.

We have publications tested in 4 applications: ELISA Development, IHC, Neutralization, Western Blot.

Showing Publications 1 - 8 of 8.

Publications using MAB243	Applications	Species
N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype Anticancer Res., 2018-04-01;38(4):2007-2014. 4/1/2018 [PMID: 29599317]
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun, 2015-02-19;6(0):6329. 2015-02-19 [PMID: 25695838] (Neutralization, Mouse)	Neutralization	Mouse
Yin H, Lo J, Kim J, Marsh E, Kim J, Ghosh A, Bulun S, Chakravarti D Expression profiling of nuclear receptors identifies key roles of NR4A subfamily in uterine fibroids. Mol Endocrinol, 2013-04-02;27(5):726-40. 2013-04-02 [PMID: 23550059] (Western Blot, Human)	Western Blot	Human
Ozturk F, Li Y, Zhu X, Guda C, Nawshad A Systematic analysis of palatal transcriptome to identify cleft palate genes within TGFbeta3-knockout mice alleles: RNA-Seq analysis of TGFbeta3 Mice. BMC Genomics, 2013-02-20;14(0):113. 2013-02-20 [PMID: 23421592] (Western Blot, Mouse)	Western Blot	Mouse
Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol. Biol. Cell, 2011-03-16;22(10):1686-98. 2011-03-16 [PMID: 21411626] (Neutralization, Canine)	Neutralization	Canine
Samira Amarir, Maria Marx, Georges Calothy Notch Signaling Activation Suppresses v-Src-Induced Transformation of Neural Cells by Restoring TGF-beta -Mediated Differentiation PLoS ONE 2010-10-22 [PMID: 21042581]
Arciniegas E, Neves CY, Candelle D, Parada D Differential versican isoforms and aggrecan expression in the chicken embryo aorta. Anat Rec A Discov Mol Cell Evol Biol, 2004-07-01;279(1):592-600. 2004-07-01 [PMID: 15224401] (IHC, Chicken)	IHC	Chicken
Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56. 2002-05-01 [PMID: 11943670] (ELISA Development, Human)	ELISA Development	Human

Array MAB243

• TGF-beta 3 Antibodies
• TGF-beta 3 ELISA Kits
• TGF-beta 3 Lysate
• TGF-beta 3 Proteins
• TGF-beta 3 ELISA
• TGF-beta 3 Proteins and Enzymes