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TGF beta 1/2/3 Antibody (1D11) [Unconjugated]

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Recombinant Human TGF‑ beta 1 (Catalog # 240‑B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of ...read more
Apoptotic cell-induced immunomodulation in mice with collagen-induced arthritis (CIA) is dependent on transforming growth factor (TGF)-beta . CIA mice that received or did not receive apoptotic cells, with or without ...read more
TGF beta 1 stimulates the expression of ECM components in human and rabbit primary cells. (A) Selected ECM mRNA transcripts were measured using qRT-PCR in human RPTEC or human IPF134 following 48 hr stimulation with ...read more
CBirTox induced expression of Foxp3 is dependent on TGF-beta but independent of RA signaling.(A) CD11c+ DCs were isolated from the spleen, MLN or LP and pulsed with 1 μg/ml of CBirTox or CBir1 peptide for 2 hours ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
1D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TGF beta 1/2/3 Antibody (1D11) [Unconjugated] Summary

Immunogen
Bovine bone-derived TGF-beta 1 and TGF-beta 2
Specificity
Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3. No cross-reactivity was observed with recombinant LAP in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGFB1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Binding Inhibition
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.25-1.25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: TGF-beta 1 Biotinylated Antibody (Catalog number BAF240)
Standard: Recombinant Human TGF-beta 1 (Catalog number 240-B)
Publications
Read Publications using
MAB1835 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF beta 1/2/3 Antibody (1D11) [Unconjugated]

  • CED
  • DPD1
  • LAP
  • TGFB
  • TGFB1
  • TGF-beta 1, 2, 3
  • TGFbeta
  • transforming growth factor, beta 1

Background

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  1. Ayala A. et al. (1992) FASEB J. 6:A1604.
  2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
  3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF beta 1/2/3 Antibody (MAB1835)(225)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, Mink, Opossum, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse.

We have publications tested in 20 applications: Activation, Agonist Activity, Bioassay, Blocking, Co-IP, Dot Blot, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunodepletion, In Vivo, Luminex Development, Neutralization, Western Blot.


Filter By Application
Activation
(1)
Agonist Activity
(1)
Bioassay
(11)
Blocking
(1)
Co-IP
(1)
Dot Blot
(1)
ELISA Capture
(2)
ELISA Development
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(7)
Functional Assay
(1)
ICC
(4)
IHC
(3)
IHC-Fr
(3)
IHC-P
(2)
Immunodepletion
(1)
In Vivo
(2)
Luminex Development
(1)
Neutralization
(119)
Western Blot
(13)
All Applications
Filter By Species
Human
(73)
Mouse
(70)
Rat
(8)
Bovine
(5)
Canine
(2)
Chicken
(1)
Hamster
(1)
Mink
(2)
Opossum
(1)
Porcine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rabbit
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 225. Show All 225 Publications.
Publications using MAB1835 Applications Species
Jeong, H;Koh, J;Kim, S;Song, SG;Lee, SH;Jeon, Y;Lee, CH;Keam, B;Lee, SH;Chung, DH;Jeon, YK; Epithelial-mesenchymal transition induced by tumor cell-intrinsic PD-L1 signaling predicts a poor response to immune checkpoint inhibitors in PD-L1-high lung cancer British journal of cancer 2024-05-10 [PMID: 38729997] (Neutralization, Human) Neutralization Human
Seller, A;Tegeler, CM;Mauermann, J;Schreiber, T;Hagelstein, I;Liebel, K;Koch, A;Heitmann, JS;Greiner, SM;Hayn, C;Dannehl, D;Engler, T;Hartkopf, AD;Hahn, M;Brucker, SY;Salih, HR;Märklin, M; Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease Outcome International journal of molecular sciences 2024-04-08 [PMID: 38612935] (Flow Cytometry, Human) Flow Cytometry Human
Kostadinova, R;Ströbel, S;Chen, L;Fiaschetti-Egli, K;Gadient, J;Pawlowska, A;Petitjean, L;Bieri, M;Thoma, E;Petitjean, M; Digital pathology with artificial intelligence analysis provides insight to the efficacy of anti-fibrotic compounds in human 3D MASH model Scientific reports 2024-03-11 [PMID: 38467661] (Bioassay, Human) Bioassay Human
Petillo, S;Sproviero, E;Loconte, L;Cuollo, L;Zingoni, A;Molfetta, R;Fionda, C;Soriani, A;Cerboni, C;Petrucci, MT;Fazio, F;Paolini, R;Santoni, A;Cippitelli, M; NEDD8-activating enzyme inhibition potentiates the anti-myeloma activity of natural killer cells Cell death & disease 2023-07-17 [PMID: 37460534] (Neutralization) Neutralization
Michielon, E;López González, M;Stolk, DA;Stolwijk, JGC;Roffel, S;Waaijman, T;Lougheed, SM;de Gruijl, TD;Gibbs, S; A Reconstructed Human Melanoma-in-Skin Model to Study Immune Modulatory and Angiogenic Mechanisms Facilitating Initial Melanoma Growth and Invasion Cancers 2023-05-20 [PMID: 37345186] (Neutralization, Human) Neutralization Human
Marvin, DL;Dijkstra, J;Zulfiqar, RM;Vermeulen, M;Ten Dijke, P;Ritsma, L; TGF-? Type I Receptor Signaling in Melanoma Liver Metastases Increases Metastatic Outgrowth International journal of molecular sciences 2023-05-12 [PMID: 37240029] (Neutralization, Mouse) Neutralization Mouse
Carneiro, CS;Hapeman, JD;Nedelcu, AM; Synergistic inter-clonal cooperation involving crosstalk, co-option and co-dependency can enhance the invasiveness of genetically distant cancer clones BMC ecology and evolution 2023-05-24 [PMID: 37226092] (Bioassay, Human) Bioassay Human
S Vondra, AL Höbler, AI Lackner, J Raffetsede, ZN Mihalic, A Vogel, L Saleh, V Kunihs, P Haslinger, M Wahrmann, H Husslein, R Oberle, J Kargl, S Haider, P Latos, G Schabbauer, M Knöfler, J Ernerudh, J Pollheimer The human placenta shapes the phenotype of decidual macrophages Cell Reports, 2023-01-10;42(1):111977. 2023-01-10 [PMID: 36640334] (Neutralization, Human) Neutralization Human
Cinzia Fionda, Silvia Ruggeri, Giuseppe Sciumè, Mattia Laffranchi, Isabella Quinti, Cinzia Milito et al. Age-dependent NK cell dysfunctions in severe COVID-19 patients Frontiers in Immunology 11/17/2022 [PMID: 36466890]
SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs Nature Immunology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456734] (ELISA Capture, Human) ELISA Capture Human
Show All 225 Publications.

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Bioinformatics

Gene Symbol TGFB1