![Western Blot: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, and Tet3. Both human (h) and mouse (m) BMMSCs expressed Tet1, Tet2, and Tet3, as assessed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04464-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg "Western Blot: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, and Tet3. Both human (h) and mouse (m) BMMSCs expressed Tet1, Tet2, and Tet3, as assessed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04464-6) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Bv, XpSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, ChIP, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TET3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 179 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 10/03/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TET3 Antibody (NBP2-20602)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: TET3 Antibody [NBP2-20602] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET3 protein stained by TET3 antibody [C3], C-term diluted at 1:400. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.](http://images.novusbio.com/fullsize/TET3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20602-img0005.jpg "Immunocytochemistry/Immunofluorescence: TET3 Antibody [NBP2-20602] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET3 protein stained by TET3 antibody [C3], C-term diluted at 1:400. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.")
![Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-20602] - Mouse colon. TET3 stained by TET3 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.](http://images.novusbio.com/fullsize/TET3-Antibody-Immunohistochemistry-Paraffin-NBP2-20602-img0010.jpg "Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-20602] - Mouse colon. TET3 stained by TET3 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.")
![Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TET3-Antibody-Western-Blot-NBP2-20602-img0012.jpg "Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/TET3-Antibody-Immunoprecipitation-NBP2-20602-img0002.jpg "Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.")
![Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p <0.001; p values calculated using two-tailed Student's t test (mean ± SD) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04464-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20602_rabbit-polyclonal-tet3-antibody-31020241530426.jpg "Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p <0.001; p values calculated using two-tailed Student's t test (mean ± SD) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04464-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP2-20602_10776.jpg)