Home » T-bet/TBX21 » T-bet/TBX21 Antibodies » T-bet/TBX21 Antibody (525803) [Janelia Fluor® 549]
Novus Biologicals products are now on bio-techne.com

T-bet/TBX21 Antibody (525803) [Janelia Fluor® 549]

 Submit a Review

Images

 
There are currently no images for T-bet/TBX21 Antibody (FAB5385I).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
525803
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC5385U-100UG
Alexa Fluor 405
IC5385V-100UG
Alexa Fluor 488
IC5385G-100UG
Alexa Fluor 532
FAB5385X
Alexa Fluor 594
IC5385T-100UG
Alexa Fluor 647
IC5385R-100UG
Alexa Fluor 700
IC5385N-100UG
Alexa Fluor 750
IC5385S-100UG
Allophycocyanin
FAB5385A
Biotin
FAB5385B
CoraFluor 1
FAB5385CL1
DyLight 350
FAB5385D
DyLight 405
FAB5385E
DyLight 488
FAB5385K
DyLight 550
FAB5385L
DyLight 594
FAB5385M
DyLight 650
FAB5385W
DyLight 680
FAB5385Y
DyLight 755
FAB5385Z
Fluorescein
IC5385F-025
Fluorescein
IC5385F
HRP
FAB5385H
Janelia Fluor 525
FAB5385JF525
Janelia Fluor 585
FAB5385JF585
Janelia Fluor 635
FAB5385JF635
Janelia Fluor 646
FAB5385J
Janelia Fluor 669
FAB5385JF669
PE
FAB5385P
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C-025
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C
Unconjugated
MAB5385
mFluor Violet 450 SE
FAB5385MFV450
mFluor Violet 500 SE
FAB5385MFV500
mFluor Violet 610 SE
FAB5385MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5385
Carrier Free
IC5385C
Carrier Free
IC5385F
Carrier Free
MAB5385
Carrier Free
MAB5385-SP
Carrier Free
IC5385C-025
Carrier Free
IC5385F-025
Carrier Free
IC5385V-100UG
Carrier Free
IC5385U-100UG
Carrier Free
IC5385T-100UG
Carrier Free
IC5385S-100UG
Carrier Free
IC5385R-100UG
Carrier Free
IC5385N-100UG
Carrier Free
IC5385G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

T-bet/TBX21 Antibody (525803) [Janelia Fluor® 549] Summary

Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T-bet.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for T-bet/TBX21 Antibody (525803) [Janelia Fluor® 549]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(451 Publications)
Species: Hu
Applications: BA
GATA-3 Antibody
[Unconjugated]
AF2605
Western Blot GATA-3 Antibody [Unconjugated]
Immunocytochemistry GATA-3 Antibody [Unconjugated]
Simple Western GATA-3 Antibody [Unconjugated]
(43 Publications)
Species: Hu
Applications: ICC, IHC, Simple Western, WB
GATA-3 Antibody
[Unconjugated]
AF2605
Western Blot GATA-3 Antibody [Unconjugated]
Immunocytochemistry GATA-3 Antibody [Unconjugated]
Simple Western GATA-3 Antibody [Unconjugated]
(43 Publications)
Species: Hu
Applications: ICC, IHC, Simple Western, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(299 Publications)
Species: Mu
Applications: ELISA
FoxP3 Antibody -
BSA Free
NB100-39002
Flow Cytometry FoxP3 Antibody - BSA Free
Flow Cytometry FoxP3 Antibody - BSA Free
Immunohistochemistry-Paraffin FoxP3 Antibody - BSA Free
(2 Reviews)
(62 Publications)
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(189 Publications)
Species: Mu
Applications: ELISA
IL-5 [HRP]
M5000
N/A IL-5 [HRP]
N/A IL-5 [HRP]
(92 Publications)
Species: Mu
Applications: ELISA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]
(147 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(652 Publications)
Species: Mu
Applications: ELISA
STAT4 [p Tyr693]
Antibody [Unconjugate ...
AF4319
Western Blot STAT4 [p Tyr693] Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: WB
EOMES Antibody
AF6166
Immunocytochemistry EOMES Antibody
Intracellular Staining by Flow Cytometry EOMES Antibody
Western Blot EOMES Antibody
(26 Publications)
Species: Hu
Applications: CyTOF-ready, ICC, ICFlow, WB
STAT1 [p Tyr701]
Antibody [Unconjugate ...
AF2894
Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]
Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
IL-17D Antibody
(246018) [Unconjugate
MAB15041
Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]
Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
STAT6 Antibody
(177C322.1) - BSA Fre
NBP2-25241
Immunocytochemistry/ Immunofluorescence STAT6 Antibody (177C322.1) - BSA Free
Western Blot STAT6 Antibody (177C322.1) - BSA Free
Immunocytochemistry/ Immunofluorescence STAT6 Antibody (177C322.1) - BSA Free
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IP, WB

Publications for T-bet/TBX21 Antibody (FAB5385I) (0)

There are no publications for T-bet/TBX21 Antibody (FAB5385I).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for T-bet/TBX21 Antibody (FAB5385I) (0)

There are no reviews for T-bet/TBX21 Antibody (FAB5385I). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (FAB5385I). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

Additional T-bet/TBX21 Products

Research Areas for T-bet/TBX21 Antibody (FAB5385I)

Find related products by research area.

Blogs on T-bet/TBX21

There are no specific blogs for T-bet/TBX21, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our T-bet/TBX21 Antibody (525803) [Janelia Fluor® 549] and receive a gift card or discount.


Bioinformatics

Gene Symbol TBX21