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T-bet/TBX21 Antibody (525803) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
525803
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC5385U-100UG
Alexa Fluor 405
IC5385V-100UG
Alexa Fluor 488
IC5385G-100UG
Alexa Fluor 532
FAB5385X
Alexa Fluor 594
IC5385T-100UG
Alexa Fluor 647
IC5385R-100UG
Alexa Fluor 700
IC5385N-100UG
Alexa Fluor 750
IC5385S-100UG
Allophycocyanin
FAB5385A
Biotin
FAB5385B
DyLight 350
FAB5385D
DyLight 405
FAB5385E
DyLight 488
FAB5385K
DyLight 550
FAB5385L
DyLight 594
FAB5385M
DyLight 650
FAB5385W
DyLight 680
FAB5385Y
DyLight 755
FAB5385Z
Fluorescein
IC5385F-025
Fluorescein
IC5385F
HRP
FAB5385H
Janelia Fluor 525
FAB5385JF525
Janelia Fluor 549
FAB5385I
Janelia Fluor 585
FAB5385JF585
Janelia Fluor 635
FAB5385JF635
Janelia Fluor 646
FAB5385J
Janelia Fluor 669
FAB5385JF669
PE
FAB5385P
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C-025
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C
Unconjugated
MAB5385
mFluor Violet 450 SE
FAB5385MFV450
mFluor Violet 500 SE
FAB5385MFV500
mFluor Violet 610 SE
FAB5385MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5385
Carrier Free
IC5385C
Carrier Free
IC5385F
Carrier Free
MAB5385
Carrier Free
MAB5385-SP
Carrier Free
IC5385C-025
Carrier Free
IC5385F-025
Carrier Free
IC5385V-100UG
Carrier Free
IC5385U-100UG
Carrier Free
IC5385T-100UG
Carrier Free
IC5385S-100UG
Carrier Free
IC5385R-100UG
Carrier Free
IC5385N-100UG
Carrier Free
IC5385G-100UG
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T-bet/TBX21 Antibody (525803) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T-bet.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for T-bet/TBX21 Antibody (525803) [CoraFluor™ 1]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (FAB5385CL1). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol TBX21