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Synaptopodin Antibody

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Western Blot: Synaptopodin Antibody [NBP1-87887] - Analysis in human skeletal muscle tissue.
Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody [NBP1-87887] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887] - Staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in striated muscle fibers.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Synaptopodin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TTPTKVYSEVHFTLAKPPSVVNRTARPFGIQAPGGTSQMERSPMLERRHFGEKAPAPQPPSLPDRSPRPQRHIMSRSPMVERRMMGQRS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYNPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Synaptopodin Protein (NBP1-87887PEP)
Publications
Read Publication using NBP1-87887.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%). Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Synaptopodin Antibody

  • KIAA1029
  • Synaptopodin
  • SYNPO

Background

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptopodin Antibody (NBP1-87887)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SYNPO