![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Western-Blot-NBP2-75624-img0005.jpg "Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, Func |
| Clone | JB51-33 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Podocin/NPHS2 aa 334-383 / 383. (SwissProt: Q9NP85 Human; SwissProt: Q91X05 Mouse; SwissProt: Q8K4G9 Rat) |
| Localization | Cell membrane. Endoplasmic reticulum. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NPHS2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Podocin/NPHS2 Antibody (NBP2-75624)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NPHS2 |
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0007.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Flow-Cytometry-NBP2-75624-img0001.jpg "Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0002.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0003.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0004.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0006.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_6775.jpg)
![Immunohistochemistry Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nephrin_AF3159_Immunohistochemistry_10004.jpg)
![Immunocytochemistry/ Immunofluorescence Nephrin Antibody [Unconjugated]](https://images.novusbio.com/images/af3159_mouse-nephrin-affinity-purified-polyclonal-ab-41202410484365.jpg)
![Immunocytochemistry Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Immunocytochemistry_6472.jpg)
![Simple Western Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Simple_Western_18959.jpg)
![Western Blot Nucleostemin Antibody [Unconjugated]](https://images.novusbio.com/images2/Nucleostemin_AF1638_Western_Blot_18960.jpg)
![Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]](https://images.novusbio.com/images2/Podocalyxin_AF1556_Immunohistochemistry_11445.jpg)
![Western Blot Podocalyxin Like Antibody [Unconjugated]](https://images.novusbio.com/images2/Podocalyxin_AF1556_Western_Blot_12581.jpg)
![Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]](https://images.novusbio.com/images/af1556_mouse-podocalyxin-affinity-purified-polyclonal-ab-41202410485429.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")