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SR-BI Antibody (SR37-06)

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Western Blot: SR-BI Antibody (SR37-06) [NBP3-11865] - Western blot analysis of SR-BI on different lysates using NBP3-11865 at 1/1,000 dilution. Positive control: Lane 1: Human liver. Lane 2: Mouse liver
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunocytochemistry staining SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using NBP3-11865. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunocytochemistry staining SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded human liver tissue using NBP3-11865. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SR-BI Antibody (SR37-06) [NBP3-11865] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using NBP3-11865. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SR37-06
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SR-BI Antibody (SR37-06) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein (Uniprot#: Q8WTV0)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SCARB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-5000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
1X TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for SR-BI Antibody (SR37-06)

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NBP3-11865) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SR-BI Products

Research Areas for SR-BI Antibody (NBP3-11865)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Bioinformatics

Gene Symbol SCARB1