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SPRY2 Antibody [Unconjugated]

 
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Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, PC-3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SPRY2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, PC-3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 ...read more
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and SH‑SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY2 at approximately 45 kDa (as indicated) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6157-SP
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SPRY2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SPRY2
Met1-Ala175
Accession # O43597
Specificity
Detects human and mouse SPRY2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPRY2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPRY2 Antibody [Unconjugated]

  • hSPRY2
  • MGC23039
  • protein sprouty homolog 2
  • sprouty (Drosophila) homolog 2
  • sprouty 2
  • sprouty homolog 2 (Drosophila)
  • SPRY2
  • spry-2

Background

SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKCδ and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPRY2
Uniprot