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Mouse SPARC ELISA Kit (Colorimetric)

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ELISA: Mouse SPARC ELISA Kit (Colorimetric) [NBP2-68075] - Samples were spiked with high concentrations of Mouse SPARC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse SPARC ELISA Kit (Colorimetric) [NBP2-68075] - Samples were spiked with high concentrations of Mouse SPARC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse SPARC ELISA Kit (Colorimetric) [NBP2-68075] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

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Mouse SPARC ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse ON in samples. No significant cross-reactivity or interference between Mouse ON and analogues was observed.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.11%
Intra-Assay
CV% < 4.79%
Spike Recovery
84-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SPARC

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse SPARC ELISA Kit (Colorimetric)

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Mouse SPARC ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol SPARC