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SPARC-like 1/SPARCL1 Antibody

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Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p < 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a ...read more
Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a ...read more
Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2836
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Catalog# & Formulation Size Price
Carrier Free
AF2836-SP
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SPARC-like 1/SPARCL1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC-like 1/SPARCL1 (R&D Systems, Catalog # 4547-SL)
Ile17-Phe650
Accession # P70663
Specificity
Detects mouse SPARC-like 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human SPARC-like 1 is observed and less than 1% cross-reactivity with recombinant mouse SPARC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPARCL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2836 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPARC-like 1/SPARCL1 Antibody

  • Hevin
  • High endothelial venule protein
  • MAST 9
  • MAST9
  • PIG33
  • proliferation-inducing protein 33
  • SC1
  • SPARC like 1
  • SPARCL1
  • SPARC-like 1 (hevin)
  • SPARC-like 1 (mast9, hevin)
  • SPARC-like 1
  • SPARC-like protein 1

Background

SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Mouse SPARCL1 contains a 16 amino acid (aa) signal sequence and a 634 aa mature region that contains four domains: a 403 aa N-terminal acidic region, a 23 aa follistatin-like domain, a 55 aa kazal-like segment and a 148 aa calcium binding domain that contains two EF hand motifs (3, 4). Mouse mature SPARCL1 shares 89%, 67%, 63%, 61%, 60%, and 58% aa identity with rat, human, equine, canine, porcine, and bovine SPARCL1, respectively. The follistatin-like, kazal-like and calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3 - 5). Some truncated forms have been reported. In mouse, a 55 kDa C‑terminal fragment is the only form in kidney and represent a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is downregulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11).

  1. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  2. Sullivan, M.M. and E.H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
  3. Girard, J.P. and T.A. Springer (1995) Immunity 2:113.
  4. Bendik, I. et al. (1998) Cancer Res. 58:626.
  5. Brekken, R.A. et al. (2004) J. Histochem. Cytochem. 52:735.
  6. Hambrock, H.O. et al. (2003) J. Biol. Chem. 278:11351.
  7. Lau, C.P. et al. (2006) J. Pathol. 210:469.
  8. Isler, S.G. et al. (2001) Int. J. Oncol. 18:521.
  9. Girard, J.P. and T.A. Springer (1996) J. Biol. Chem. 271:4511.
  10. Gongidi, V. et al. (2004) Neuron 41:57.
  11. Sullivan, M.M. et al. (2006) J. Biol. Chem. 281:27621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SPARC-like 1/SPARCL1 Antibody
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Publications for SPARC-like 1/SPARCL1 Antibody (AF2836)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, Immunohistochemistry, RNAScope, Western Blot.


Filter By Application
ICC
(2)
IHC
(7)
Immunohistochemistry
(1)
RNAScope
(1)
Western Blot
(7)
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Human
(1)
Mouse
(12)
Rat
(1)
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(1)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF2836 Applications Species
Le, AD;Fu, M;Kumar, R;Zacharias, G;Garcia, ADR; Astrocyte modulation of synaptic plasticity mediated by activity-dependent Sonic hedgehog signaling bioRxiv : the preprint server for biology 2024-06-13 [PMID: 38915525] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Niebergall, EB;Weekley, D;Mazur, A;Olszewski, NA;DeSchepper, KM;Radant, N;Vijay, AS;Risher, WC; Abnormal Morphology and Synaptogenic Signaling in Astrocytes Following Prenatal Opioid Exposure Cells 2024-05-14 [PMID: 38786059] (Western Blot, Rat) Western Blot Rat
AB Alfonso, V Pomerleau, VR Nicolás, J Raisch, CM Jurkovic, FM Boisvert, N Perreault Comprehensive Profiling of Early Neoplastic Gastric Microenvironment Modifications and Biodynamics in Impaired BMP-Signaling FoxL1+-Telocytes Biomedicines, 2022-12-22;11(1):. 2022-12-22 [PMID: 36672527] (Western Blot, Mouse) Western Blot Mouse
Y Wang, S Zhang, Z Lan, V Doan, B Kim, S Liu, M Zhu, VL Hull, S Rihani, CL Zhang, JA Gray, F Guo SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice Cell Reports, 2022-12-20;41(12):111842. 2022-12-20 [PMID: 36543123] (Western Blot, Mouse) Western Blot Mouse
GP Shelkar, PJ Gandhi, J Liu, SM Dravid Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens Science Advances, 2022-07-22;8(29):eabo6574. 2022-07-22 [PMID: 35867797] (Western Blot, Mouse) Western Blot Mouse
Wu X, Sosunov AA, Lado W et al. Synaptic hyperexcitability of cytomegalic pyramidal neurons contributes to epileptogenesis in tuberous sclerosis complex Cell reports 7/19/2022 [PMID: 35858542]
T Taketomi, T Yasuda, R Morita, J Kim, Y Shigeta, C Eroglu, R Harada, F Tsuruta Autism-associated mutation in Hevin/Sparcl1 induces endoplasmic reticulum stress through structural instability Scientific Reports, 2022-07-13;12(1):11891. 2022-07-13 [PMID: 35831437] (ICC, Western Blot, Human) ICC, Western Blot Human
I Osokine, J Siewiera, D Rideaux, S Ma, T Tsukui, A Erlebacher Gene silencing by EZH2 suppresses TGF-beta activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface Cell Reports, 2022-02-01;38(5):110329. 2022-02-01 [PMID: 35108527] (IHC, Mouse) IHC Mouse
T Udagawa, PJ Atkinson, B Milon, JM Abitbol, Y Song, M Sperber, E Huarcaya N, M Scheibinge, R Elkon, R Hertzano, AG Cheng Lineage-tracing and translatomic analysis of damage-inducible mitotic cochlear progenitors identifies candidate genes regulating regeneration PloS Biology, 2021-11-10;19(11):e3001445. 2021-11-10 [PMID: 34758021] (IHC, Mouse) IHC Mouse
TA Jan, Y Eltawil, AH Ling, L Chen, DC Ellwanger, S Heller, AG Cheng Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics Cell Reports, 2021-07-13;36(2):109358. 2021-07-13 [PMID: 34260939] (ICC, IHC, RNAScope, Mouse) ICC, IHC, RNAScope Mouse
Show All 17 Publications.

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Bioinformatics

Gene Symbol SPARCL1
Uniprot