SPARC Antibodies
We offer SPARC Antibodies for use in common research applications: ELISA, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Sandwich ELISA, Simple Western, Western Blot. Each SPARC Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SPARC Antibodies can be used in a variety of model species: Human, Mouse, Primate. Use the list below to choose the SPARC Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SPARC polyclonal antibodies and browse our SPARC monoclonal antibody catalog.
Alternate Names for SPARC Antibodies
anti-SPARC antibody, anti-Basement-membrane protein 40 antibody, anti-BM-40 antibody, anti-ONcysteine-rich protein antibody, anti-osteonectin antibody, anti-Secreted protein acidic and rich in cysteine antibody, anti-secreted protein, acidic, cysteine-rich (osteonectin) antibody
18 Results for "sparc" in Primary Antibodies
![Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis using Anti-SPARC antibody NBP1-80972 (A) shows similar pattern to independent antibody NBP1-80971 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SPARC Antibody [NBP1-80972] - Staining SPARC in MG63 cells using anti-SPARC antibody. Cell were fixed in 4% Paraformaldehyde (20 min, RT); and permeabilized in 0,2% Triton (15 min, RT). Image from verified customer review.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80972-img0006.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80972] - Staining of human placenta shows strong positivity in endothelial cells and fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80972-img0011.jpg)
![Western Blot: SPARC Antibody [NBP1-80972] - Analysis of SPARC MC3T3 (mouse), MG63 (human) cell lysates and conditions media using anti-SPARC antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0005.jpg)
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis using Anti-SPARC antibody NBP1-80971 (A) shows similar pattern to independent antibody NBP1-80972 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0010.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human placenta shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0013.jpg)
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0009.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0011.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0012.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human skin shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0014.jpg)
![Western Blot: SPARC Antibody [NBP2-27358] - Analysis of human A375 lysate probed with SPARC antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP2-27358-img0001.jpg "Western Blot: SPARC Antibody [NBP2-27358] - Analysis of human A375 lysate probed with SPARC antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358] - FFPE human placenta tissue stained with SPARC antibody, peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP2-27358-img0002.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody (MM0557-8N38) [NBP2-11913] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11913. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/SPARC-Antibody-MM0557-8N38-Immunohistochemistry-Paraffin-NBP2-11913-img0001.jpg "Immunohistochemistry-Paraffin: SPARC Antibody (MM0557-8N38) [NBP2-11913] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11913. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.")
![Western Blot: SPARC Antibody (001) [NBP2-90612] - Anti-SPARC rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/SPARC-Antibody-001-Western-Blot-NBP2-90612-img0003.jpg "Western Blot: SPARC Antibody (001) [NBP2-90612] - Anti-SPARC rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 40 kDa")
![Immunoprecipitation: SPARC Antibody (001) [NBP2-90612] - Mouse SPARC was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 0.5 ul anti-Mouse SPARC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse SPARC rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/SPARC-Antibody-001-Immunoprecipitation-NBP2-90612-img0004.jpg)
![Immunohistochemistry: SPARC Antibody (PD00-57) [NBP3-32974] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SPARC antibody (NBP3-32974) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32974) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32974_rabbit-sparc-mab-pd00-57-298202414333618.jpg "Immunohistochemistry: SPARC Antibody (PD00-57) [NBP3-32974] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SPARC antibody (NBP3-32974) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32974) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
