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Recombinant Human SPARC Protein

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SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]
SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Human SPARC Protein Summary

Description
A single non-glycosylated polypeptide chain containing 286 amino acids corresponding to SPARC Source: E. coli

Uniprot ID: P09486

Amino Acid Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

Details of Functionality
SPARC protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SPARC
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of SPARC as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>98%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human SPARC Protein

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SPARC