| Reactivity | HuSpecies Glossary |
| Applications | PAGE, Bioactivity |
| Format | Carrier-Free |
| Concentration | LYOPH |
| Description | A single non-glycosylated polypeptide chain containing 314 amino acids corresponding to SPAR, with expression vector sequence containing 6x His tag Source: E. coli Uniprot ID: P09486 Amino Acid Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI |
| Details of Functionality | SPARC protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | SPARC |
| Purity | >95%, by SDS-PAGE and HPLC |
| Endotoxin Note | Less than 1 EU/ug of SPARC as determined by LAL method. |
| Dilutions |
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| Theoretical MW | 36.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
| Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95%, by SDS-PAGE and HPLC |
| Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
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