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SPARC Antibody (122511) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, CyTOF-ready, Flow
Clone
122511
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC941U-100UG
Alexa Fluor 405
IC941V-100UG
Alexa Fluor 488
IC941G-100UG
Alexa Fluor 532
FAB941X
Alexa Fluor 594
IC941T-100UG
Alexa Fluor 647
IC941R-100UG
Alexa Fluor 700
IC941N-100UG
Alexa Fluor 750
IC941S-100UG
Allophycocyanin
FAB941A
Biotin
FAB941B
CoraFluor 1
FAB941CL1
DyLight 350
FAB941D
DyLight 405
FAB941E
DyLight 488
FAB941K
DyLight 550
FAB941L
DyLight 594
FAB941M
DyLight 650
FAB941W
DyLight 680
FAB941Y
FITC
FAB941F
HRP
FAB941H
Janelia Fluor 525
FAB941JF525
Janelia Fluor 549
FAB941I
Janelia Fluor 646
FAB941J
Janelia Fluor 669
FAB941JF669
PerCP
FAB941C
Unconjugated
MAB941-100
mFluor Violet 450 SE
FAB941MFV450
mFluor Violet 500 SE
FAB941MFV500
mFluor Violet 610 SE
FAB941MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB941-100
Carrier Free
MAB941-SP
Carrier Free
MAB941-100
Carrier Free
MAB941-500
Carrier Free
IC941V-100UG
Carrier Free
IC941R-100UG
Carrier Free
IC941U-100UG
Carrier Free
IC941N-100UG
Carrier Free
IC941G-100UG
Carrier Free
IC941T-100UG
Carrier Free
IC941S-100UG
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SPARC Antibody (122511) [DyLight 755] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human SPARC
Ala18-Ile303
Accession # P09486
Specificity
Detects human SPARC/Osteonectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SPARC/Osteonectin is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPARC
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SPARC Antibody (122511) [DyLight 755]

  • Basement-membrane protein 40
  • BM-40
  • ONcysteine-rich protein
  • Osteonectin
  • Secreted protein acidic and rich in cysteine
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(551 Publications)
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SPARC Antibody
(122511) [DyLight 755
FAB941Z
SPARC Antibody (122511) [DyLight 755]
Species: Hu
Applications: ELISA, IHC, CyTOF-ready, Flow

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPARC