![Western Blot: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Western blot analysis of HepG2 cell lysate using SOX9 antibody (SOX9/2398).](http://images.novusbio.com/fullsize/SOX9-Antibody-SOX9-2398-Western-Blot-NBP3-07659-img0003.jpg "Western Blot: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Western blot analysis of HepG2 cell lysate using SOX9 antibody (SOX9/2398).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | SOX9/2398 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08399) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary) (Uniprot: P48436) |
| Localization | Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SOX9 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human pancreas stained with SOX9 Antibody (SOX9/2398). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SOX9-Antibody-SOX9-2398-Immunohistochemistry-Paraffin-NBP3-07659-img0004.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human pancreas stained with SOX9 Antibody (SOX9/2398). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human Pancreas stained with SOX9 Mouse Monoclonal Antibody (SOX9/2398).](http://images.novusbio.com/fullsize/SOX9-Antibody-SOX9-2398-Immunohistochemistry-Paraffin-NBP3-07659-img0002.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Formalin-fixed, paraffin-embedded human Pancreas stained with SOX9 Mouse Monoclonal Antibody (SOX9/2398).")
![Protein Array: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX9 Mouse Monoclonal Antibody (SOX9/2398).](http://images.novusbio.com/fullsize/SOX9-Antibody-SOX9-2398-Protein-Array-NBP3-07659-img0001.jpg "Protein Array: SOX9 Antibody (SOX9/2398) [NBP3-07659] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX9 Mouse Monoclonal Antibody (SOX9/2398).")
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