Novus Biologicals products are now on bio-techne.com

Sort By:

Products (8)

page of 1

SOX1 Products By Gene Name

8 Results for "SOX1"

AF3369

SOX1 Antibody [Unconjugated]

(126 Publications)

100 μg

AF3369

Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

SOX1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 was detected in immersion fixed rat cortical stem cells (NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neuron. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells andiBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, DQuantification of percentage of SOX1+ (C) and SOX2+ (D) cells in culture. The efficiency of neural induction is more than 99% in FKRP‐ and corrected‐iPSC (5D17, 5D23, and 3B17) lines. Data are mean ± s.d. n = 4 technical replicates.E, FFKRP‐ and corrected‐NSC lines can be further differentiated to cortical neural progenitor cells, expressing PAX6, OTX2, and vimentin.G–IQuantification of percentage of PAX6+ (G) and OTX2+ (H) cells in culture. About 91‐98% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express PAX6 (G). About 93‐96% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express OTX2 (H). Of the OTX2+ population, about 60‐67% cells are also Ki67+ cycling progenitors (I). Data are mean ± s.d. n = 4 technical replicates.J, KGlutamatergic projection neurons derived from FKRP and corrected (5D17, 5D23, and 3B17) progenitor cells. The vast majority of neurons contain vGlut1+ punctae in their neurites (labeled by Tuj1). Right panels are enlarged images from the insets of left panels.Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, DQuantification of percentage of SOX1+ (C) and SOX2+ (D) cells in culture. The efficiency of neural induction is more than 99% in FKRP‐ and corrected‐iPSC (5D17, 5D23, and 3B17) lines. Data are mean ± s.d. n = 4 technical replicates.E, FFKRP‐ and corrected‐NSC lines can be further differentiated to cortical neural progenitor cells, expressing PAX6, OTX2, and vimentin.G–IQuantification of percentage of PAX6+ (G) and OTX2+ (H) cells in culture. About 91‐98% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express PAX6 (G). About 93‐96% of cells derived from FKRP, 5D17, 5D23, and 3B17 NSC lines express OTX2 (H). Of the OTX2+ population, about 60‐67% cells are also Ki67+ cycling progenitors (I). Data are mean ± s.d. n = 4 technical replicates.J, KGlutamatergic projection neurons derived from FKRP and corrected (5D17, 5D23, and 3B17) progenitor cells. The vast majority of neurons contain vGlut1+ punctae in their neurites (labeled by Tuj1). Right panels are enlarged images from the insets of left panels.Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). Scale bar, 1 mm.(B) Prosencephalon labeled for NSUN2 and SOX1. Region in squares are magnified in (b′) and (b″). Arrows indicate NSUN2-positive cells. Scale bar, 100 μm.(C–F) Bright-field image (C) and immunofluorescence (D–F) of AF22 (upper panels) and Sai1 (lower panels) cells labeled for Nestin (D), SOX2 (E), and beta III-tubulin (F). Scale bar, 50 μm.(G and H) NES cells co-labeled for NSUN2 and Nestin (NES) (G) or SOX1 (H).(I) Differentiation protocol.(J–L) Differentiated AF22 and Sai1 cells (day 15) labeled for Nestin (NES; J), SOX2 (K), and beta III-tubulin (L). Scale bars: 50 μm.(M) Western blot for NSUN2, beta III-tubulin (TUBB3), GFAP, SOX2, and Nestin during differentiation (days). alpha -Tubulin served as loading control.Nuclei are counterstained with DAPI (A, B, D–F, J–L). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213671116302764), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). Scale bar, 1 mm.(B) Prosencephalon labeled for NSUN2 and SOX1. Region in squares are magnified in (b′) and (b″). Arrows indicate NSUN2-positive cells. Scale bar, 100 μm.(C–F) Bright-field image (C) and immunofluorescence (D–F) of AF22 (upper panels) and Sai1 (lower panels) cells labeled for Nestin (D), SOX2 (E), and beta III-tubulin (F). Scale bar, 50 μm.(G and H) NES cells co-labeled for NSUN2 and Nestin (NES) (G) or SOX1 (H).(I) Differentiation protocol.(J–L) Differentiated AF22 and Sai1 cells (day 15) labeled for Nestin (NES; J), SOX2 (K), and beta III-tubulin (L). Scale bars: 50 μm.(M) Western blot for NSUN2, beta III-tubulin (TUBB3), GFAP, SOX2, and Nestin during differentiation (days). alpha -Tubulin served as loading control.Nuclei are counterstained with DAPI (A, B, D–F, J–L). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2213671116302764), licensed under a CC-BY license. Not internally tested by R&D Systems.

GPI anchored proteins are required for neural differentiation.(A). Representative example of images of hiPSC-derived EBs and EB-derived rosettes during neural differentiation. Neural induction and rosette formation upon neural induction was assessed in three cell lines using a serum-free EB generation method. On day 2 (left) of hiPSC-derived EBs from PIGAwt, PIGAc.1234C>T, and PIGAnull after forced aggregation (20X magnification, scale bar is 50μm). On day 4 (middle), single homogeneous hiPSC-EBs collected were pooled in a 10 cm plate (4X magnification, scale bar is 100μm). On day 11 (right), neuroepithelial cells appeared and neural tube-like rosettes formed (EB-derived rosettes) and scale bar is 50μm. (B). Neural induction rates from EB-derived rosettes. The percentage of EB derived rosettes was 88.8% ± 4.6, 75.5% ± 9.8 and 68.4% ± 6.9 for PIGAwt, PIGAc.1234C>T, and PIGAnull, respectively. PIGAwt versus PIGAc.1234C>T (p>0.05, NS) and PIGAwt versus PIGAnull (*pT compared to PIGAwt. (F). Graphs depict the percentage of positive cells for SOX-1 (left) and Pax6 (right) in hNPCs derived from PIGAwt, PIGAc.1234C>T, and PIGAnull hiPSC lines. The hNPCs derived from the PIGAnull hiPSCs showed significantly decreased expression of SOX1 and PAX6. Similar levels of SOX1 and PAX6 were expressed in hNPCs from PIGAwt and PIGAc.1234C>T. All values represent mean ± SD. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3369-SP

compare

(select up to 3 products from this page to compare)

NBP2-24486

SOX1 Antibody

(1 Review)

(1 Publication)

0.1 mg

NBP2-24486

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis of extracts from mouse brain using SOX1 antibody (NBP2-24486, 1:100). Image from verified customer review.

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis in D3 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24486 at 1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody (Novus, 20301) and PicoTect ECL substrate solution (Novus, 10087K) were used.

Reactivity:

Hu, Mu, Eq, Ma-Op, Pm, Xp, Rt, Bv, Ca, Fe

Species Glossary

Reactivity: Hu, Mu, Eq, Ma-Op, Pm, Xp, Rt, Bv, Ca, Fe

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6446A

Alt Catalog# IMG-6446A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24486AF350
Alexa Fluor 405	NBP2-24486AF405
Alexa Fluor 488	NBP2-24486AF488
Alexa Fluor 532	NBP2-24486AF532
Alexa Fluor 594	NBP2-24486AF594
Alexa Fluor 647	NBP2-24486AF647
Alexa Fluor 700	NBP2-24486AF700
Alexa Fluor 750	NBP2-24486AF750
Allophycocyanin	NBP2-24486APC
Biotin	NBP2-24486B
DyLight 350	NBP2-24486UV
DyLight 405	NBP2-24486V
DyLight 488	NBP2-24486G
DyLight 550	NBP2-24486R
DyLight 594	NBP2-24486DL594
DyLight 650	NBP2-24486C
DyLight 680	NBP2-24486FR
DyLight 755	NBP2-24486IR
HRP	NBP2-24486H
Janelia Fluor 549	NBP2-24486JF549
Janelia Fluor 646	NBP2-24486JF646
PE	NBP2-24486PE
PerCP	NBP2-24486PCP
mFluor Violet 450 SE	NBP2-24486MFV450
mFluor Violet 500 SE	NBP2-24486MFV500
mFluor Violet 610 SE	NBP2-24486MFV610

compare

(select up to 3 products from this page to compare)

NBP2-24466

SOX1 Antibody

0.1 mg

NBP2-24466

Western Blot: SOX1 Antibody [NBP2-24466] - Western blot analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse brain lysate using NBP2-24466 at 0.5 ug/ml, 0.5 ug/ml and 2 ug/ml respectively.

Reactivity:

Hu, Mu, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Ca, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6471A

Alt Catalog# IMG-6471A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-67864

SOX1 Antibody (JJ20-40)

100 ul

NBP2-67864

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864] - Western blot analysis of SOX1 on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra

Immunocytochemistry/Immunofluorescence: SOX1 Antibody (JJ20-40) [NBP2-67864] - Staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOX1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SOX1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-16153

SOX1 Antibody - Azide and BSA Free

100 ul

NBP3-16153

Western Blot: SOX1 Antibody [NBP3-16153] - Western blot analysis of extracts of various cell lines, using SOX1 antibody (NBP3-16153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: SOX1 Antibody [NBP3-16153] - Immunohistochemistry of paraffin-embedded mouse embryos using SOX1 Rabbit pAb (NBP3-16153) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-16153-20ul

compare

(select up to 3 products from this page to compare)

NBP3-10486

SOX1 Antibody

100 ul

NBP3-10486

Western Blot: SOX1 Antibody [NBP3-10486] - Western blot analysis using NBP3-10486 on Human Jurkat as a positive control. Antibody Titration: 0.2-1 ug/ml

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-16619

SOX1 Antibody (9L8Z4)

100 ul

NBP3-16619

Western Blot: SOX1 Antibody (9L8Z4) [NBP3-16619] - Western blot analysis of extracts of A-549 cells, using SOX1 Rabbit mAb (NBP3-16619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBL1-16343

SOX1 Overexpression Lysate

0.1 mg

NBL1-16343

Western Blot: SOX1 Overexpression Lysate (Adult Normal) [NBL1-16343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOX1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

©2024 Novus Biologicals, All Rights Reserved.