![Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).](http://images.novusbio.com/fullsize/Smad2-[p-Thr220]-Antibody-Western-Blot-NBP1-04982-img0004.jpg "Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2 (NP_001003652.1). |
| Modification | p Thr220 |
| Specificity | Detects endogenous levels of Smad2 only when phosphorylated at threonine 220. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMAD2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P reactivity reported in scientific literature (PMID: 23370707). |
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| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol |
| Preservative | 0.01% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-04982 | Applications | Species |
|---|---|---|
| Lee JM, Lee DJ, Bae SC, Jung HS. Abnormal liver differentiation and excessive angiogenesis in mice lacking Runx3. Histochem Cell Biol 2013-01-31 [PMID: 23370707] (IHC-P, Mouse) | IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Smad2 Antibody (NBP1-04982)Find related products by research area.
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TGF-beta for treating degenerative intervertebral disc disease By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain... Read full blog post. |
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