![Western Blot: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Western-Blot-NBP2-74153-img0001.jpg "Western Blot: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI4F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human SH2D2A(NP_003966) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SH2D2A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 42.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
Secondary Antibodies |
Isotype Controls |
Research Areas for SH2D2A Antibody (NBP2-74153)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SH2D2A |
![Immunocytochemistry/Immunofluorescence: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A .](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74153-img0003.jpg "Immunocytochemistry/Immunofluorescence: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A .")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0010.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody.")
![Flow Cytometry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SH2D2A antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74153-img0002.jpg "Flow Cytometry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SH2D2A antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0004.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0005.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0006.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0007.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0008.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human lymphoma tissue using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0009.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human lymphoma tissue using anti-SH2D2A mouse monoclonal antibody.")
![Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human tonsil using anti-SH2D2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH2D2A-Antibody-OTI4F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74153-img0011.jpg "Immunohistochemistry: SH2D2A Antibody (OTI4F3) - Azide and BSA Free [NBP2-74153] - Staining of paraffin-embedded Human tonsil using anti-SH2D2A mouse monoclonal antibody.")
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![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
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![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")