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SGLT2/SLC5A2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Analysis in human kidney and endometrium tissues. Corresponding SLC5A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - SGLT2/SLC5A2 used at 1:50 on a tenrec kidney. SGLT2/SLC5A2 antibody was used on paraffin-embedded kidney tissue at a concentration of 20ug/mL and left ...read more
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Staining of human kidney shows moderate positivity in apical membrane in cells in tubules.
Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP1-92384] - Analysis of SGLT2/SLC5A2 antibody on Canine kidney tissue. HIER was done in pH 6 (Citrate Buffer) for two hours at 75C, and primary antibody was put on ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Tn, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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SGLT2/SLC5A2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FHEVGGYSGLFDKYLGAATSLTVSEDPAVGNISSFCYRPRPDSYHLL
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC5A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reactivity reported in scientific literature (PMID: 25894829).
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot Reactivity reported in scientific literature (PMID: 25894829).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SGLT2/SLC5A2 Recombinant Protein Antigen (NBP1-92384PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-92384 in the following applications:

Publications
Read Publications using
NBP1-92384 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33712686). Canine reactivity reported from a verified customer review. Tenrec reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SGLT2/SLC5A2 Antibody

  • Low affinity sodium-glucose cotransporter
  • Na(+)/glucose cotransporter 2
  • SGLT2
  • SGLT2sodium/glucose cotransporter 2
  • SLC5A2
  • solute carrier family 5 (sodium/glucose cotransporter), member 2
  • solute carrier family 5 (sodium/glucose transporter), member 2
  • Solute carrier family 5 member 2

Background

The SGLT2 gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-92384
Species: Hu, Mu, Ca, Tn, Rt
Applications: WB, ICC/IF, IHC

Publications for SGLT2/SLC5A2 Antibody (NBP1-92384)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(2)
IHC
(3)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-92384 Applications Species
Shim B, Stokum JA, Moyer M et al. Canagliflozin, an Inhibitor of the Na(+)-Coupled D-Glucose Cotransporter, SGLT2, Inhibits Astrocyte Swelling and Brain Swelling in Cerebral Ischemia Cells 2023-09-06 [PMID: 37759444]
Yu H, Wang M, Yu J et al. Evaluation of the efficacy of Abelmoschus manihot (L.) on diabetic nephropathy by analyzing biomarkers in the glomeruli and proximal and distal convoluted tubules of the kidneys Frontiers in Pharmacology 2023-08-01 [PMID: 37587982] (IHC) IHC
Herat LY, Matthews JR, Hibbs M et al. SGLT1/2 inhibition improves glycemic control and multi-organ protection in type 1 diabetes iScience 2023-08-18 [PMID: 37520739] (IHC) IHC
Schwertheim S, Alhardan M, Manka PP et al. Higher pNRF2, SOCS3, IRF3, and RIG1 Tissue Protein Expression in NASH Patients versus NAFL Patients: pNRF2 Expression Is Concomitantly Associated with Elevated Fasting Glucose Levels Journal of Personalized Medicine 2023-07-18 [PMID: 37511764] (IHC) IHC
Berghaus C, Groh AC, Breljak D et al. Impact of Pals1 on Expression and Localization of Transporters Belonging to the Solute Carrier Family Frontiers in Molecular Biosciences 2022-02-16 [PMID: 35252349]
Du J, Gu J, Deng J et al. The expression and survival significance of sodium glucose transporters in pancreatic cancer BMC cancer 2022-01-28 [PMID: 35090421] (IHC-P, Human) IHC-P Human
Mitsuhata Y, Abe T, Misaki K et al. Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3 Scientific reports 2021-03-12 [PMID: 33712686] (Mouse) Mouse
Chiba Y, Murakami R, Matsumoto K et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium International Journal of Molecular Sciences 2020-09-30 [PMID: 33008107] (IF/IHC, Human) IF/IHC Human
Chiba Y, Sugiyama Y, Nishi N et al. Sodium/glucose cotransporter 2 is expressed in choroid plexus epithelial cells and ependymal cells in human and mouse brains Neuropathology 2020-10-01 [PMID: 32488949] (IHC-P, Human) IHC-P Human
Dai C, Walker J. T, et al. Dapagliflozin Does Not Directly Affect Human alpha or beta Cells. Endocrinology 2020-08-01 [PMID: 32428240] (IF/IHC, Mouse) IF/IHC Mouse
Show All 12 Publications.

Reviews for SGLT2/SLC5A2 Antibody (NBP1-92384) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Canine, Tenrec.

Reviews using NBP1-92384:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Canine
(1)
Tenrec
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Vojtech Gabriel
IHC-P Tenrec 05/11/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC Sample Tested
SpeciesTenrec
Lot000023192
  4
reviewed by:
Verified Customer
IHC-P Canine 03/23/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedKidney tissue
SpeciesCanine
Lot000023192

Comments

CommentsCanine Kidney. HIER was done in pH 6 (Citrate Buffer) for two hours at 75C, and primary antibody was put on at 1:100 overnight at 4C. Image was taken at 40X.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SGLT2/SLC5A2 Antibody (NBP1-92384) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Vojtech Gabriel
05/11/2022
Application: IHC-P
Species: Tenrec

Verified Customer
03/23/2022
Application: IHC-P
Species: Canine

Bioinformatics

Gene Symbol SLC5A2