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SETDB1 Antibody (4A3)

Images

 
Western Blot: SETDB1 Antibody (4A3) [H00009869-M07] - Analysis of SETDB1 expression in SW-13 (Cat # L005V1).
Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (4A3) [H00009869-M07] - Analysis of monoclonal antibody to SETDB1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: SETDB1 Antibody (4A3) [H00009869-M07] - Detection limit for recombinant GST tagged SETDB1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SETDB1 Antibody (4A3) Summary

Immunogen
SETDB1 (NP_036564, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSLPGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCVQQRKKQLAELETWVIQKESEVAHVDQLFDDASRAVTNCESLVKDFY
Specificity
SETDB1 - SET domain, bifurcated 1 (4A3)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SETDB1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SETDB1 Antibody (4A3)

  • ERG-associated protein with SET domain
  • ESETEC 2.1.1.43
  • H3-K9-HMTase 4
  • Histone H3-K9 methyltransferase 4
  • histone-lysine N-methyltransferase SETDB1
  • histone-lysine N-methyltransferase, H3lysine-9 specific 4
  • KG1T
  • KIAA0067H3-K9-HMTase4
  • KMT1EERG-associated protein with a SET domain, ESET
  • Lysine N-methyltransferase 1E
  • SET domain bifurcated 1
  • SET domain, bifurcated 1

Background

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SETDB1 Antibody (H00009869-M07) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SETDB1
Entrez
Uniprot