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SEC3 Antibody

Images

 
Western Blot: SEC3 Antibody [NBP1-89957] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: SEC3 Antibody [NBP1-89957] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SEC3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FKLQQHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSEKDMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SEC3 Protein (NBP1-89957PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SEC3 Antibody

  • BM-102
  • exocyst complex component 1
  • Exocyst complex component Sec3
  • FLJ10893
  • SEC3L1
  • SEC3-like 1 (S. cerevisiae)
  • SEC3-like 1
  • SEC3P
  • SEC3Sec3p

Background

The EXOC1 (also known as SEC3) gene encodes an exocyst complex component 1 protein, that in isoform 1 is 894 amino acids long and nearly 102 kDA and in isoform 2 is 879 amino acids long and 100 kDA. The EXOC1 gene functions in the docking of exocytic vesicles with fusion sites on the plasma membrane. EXOC1 participates in the insulin pathway, Arf6 trafficking events, exocyst complex formation, and the metabolism of proteins through interacts with genes EXOC4, PKP3, LUC7L2, CDC5L, and C7orf55-LUC7L2. EXOC1 has been linked to toxic shock syndrome, schizophrenia, and mucopolysaccharidosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EXOC1