![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, and SEC8), or in one allele (SEC3 and SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-07467-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Western-Blot-NBP1-97500-img0005.jpg "Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, and SEC8), or in one allele (SEC3 and SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-07467-5) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Ha, Pm, Rb, Sh, YeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, WB |
| Clone | 9H5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant rat Sec6. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EXOC3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Detects a band of ~87kDa by Western blot. Use in Immunoblotting reported in scientific literature (PMID 28358000). |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EXOC3 |
![Immunohistochemistry-Paraffin: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10ug/ml.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Immunohistochemistry-Paraffin-NBP1-97500-img0003.jpg "Immunohistochemistry-Paraffin: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10ug/ml.")
![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of pig ESK-4 cell lysate.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Western-Blot-NBP1-97500-img0004.jpg "Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of pig ESK-4 cell lysate.")
![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment & validation of tagged exocyst subunit cell lines. a Schematic showing a region of subunit gene targeted by sgRNA & tags to be inserted C-terminally, in-frame with the coding region. b Strategy to isolate CRISPR/Cas9-mediated sfGFP-tagged clones of exocyst subunits in NMuMG cells. c Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, & SEC8), or in one allele (SEC3 & SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. d Confocal images of live wild-type NMuMG cells & endogenously tagged SEC3-GFP, SEC5-GFP, SEC6-GFP, SEC8-GFP, & EXO70-GFP cell lines. Scale bar = 10 μm. e Protein–protein interaction heat map for endogenous SEC3-GFP, SEC5-GFP, & EXO70-GFP pulldown using GFP-Trap nanobodies & MS. Yellow indicates baits, blue the protein identified with high confidence, & gray denotes preys that were not identified. The experiments were repeated three times for EXO70-GFP & twice for SEC3-GFP & SEC5-GFP. All experiments were also partially confirmed by IP–WB in at least three independent experiments, with similar results. f Western blot analysis to assess the relative abundance of exocyst subunits fused to sfGFP, using anti-GFP antibodies (SEC3 & SEC6 quantifications corrected for heterozygosity). GAPDH was the loading control. Y axis shows the ratio of GFP/GAPDH. Quantification shows pooled data from three experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510181), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97500_mouse-monoclonal-sec6-antibody-9h5-310202416114734.jpg "Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment & validation of tagged exocyst subunit cell lines. a Schematic showing a region of subunit gene targeted by sgRNA & tags to be inserted C-terminally, in-frame with the coding region. b Strategy to isolate CRISPR/Cas9-mediated sfGFP-tagged clones of exocyst subunits in NMuMG cells. c Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, & SEC8), or in one allele (SEC3 & SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. d Confocal images of live wild-type NMuMG cells & endogenously tagged SEC3-GFP, SEC5-GFP, SEC6-GFP, SEC8-GFP, & EXO70-GFP cell lines. Scale bar = 10 μm. e Protein–protein interaction heat map for endogenous SEC3-GFP, SEC5-GFP, & EXO70-GFP pulldown using GFP-Trap nanobodies & MS. Yellow indicates baits, blue the protein identified with high confidence, & gray denotes preys that were not identified. The experiments were repeated three times for EXO70-GFP & twice for SEC3-GFP & SEC5-GFP. All experiments were also partially confirmed by IP–WB in at least three independent experiments, with similar results. f Western blot analysis to assess the relative abundance of exocyst subunits fused to sfGFP, using anti-GFP antibodies (SEC3 & SEC6 quantifications corrected for heterozygosity). GAPDH was the loading control. Y axis shows the ratio of GFP/GAPDH. Quantification shows pooled data from three experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510181), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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