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S100A9 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Analysis in human esophagus and stomach tissues. Corresponding S100A9 RNA-seq data are presented for the same tissues.
Western Blot: S100A9 Antibody [NBP1-89360] - Analysis in human cell line SK-BR-3.
Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human stomach shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human esophagus shows moderate to strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human bone marrow shows strong cytoplasmic positivity in a subset of hematopoietic cells.
Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human skin shows moderate to strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human duodenumshows strong cytoplasmic positivity in peripheral leukocytes.
Simple Western: S100A9 Antibody [NBP1-89360] - Simple Western lane view shows a specific band for S100A9 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: S100A9 Antibody [NBP1-89360] - Electropherogram image(s) of corresponding Simple Western lane view. S100A9 antibody was used at 1:20 dilution on h. Tonsil lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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S100A9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S100A9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
S100A9 Recombinant Protein Antigen (NBP1-89360PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for S100A9 Antibody

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

FAQs for S100A9 Antibody (NBP1-89360). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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Bioinformatics

Gene Symbol S100A9