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S100A10 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) ...read more
S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

S100A10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse S100A10
Pro2-Lys97
Accession # P08207
Specificity
Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100A10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2377 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A10 Antibody [Unconjugated]

  • annexin II ligand, calpactin I, light polypeptide
  • ANX2L
  • ANX2LG
  • CAL1LGP11,42C
  • Calpactin I light chain
  • Calpactin-1 light chain
  • Cellular ligand of annexin II
  • CLP11Ca[1]
  • MGC111133
  • p10 protein
  • p10
  • P11
  • protein S100-A10
  • S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
  • S100 calcium binding protein A10
  • S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
  • S100 calcium-binding protein A10
  • S100A10

Background

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as well as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A10 Antibody (AF2377)(20)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, IHC-F, Western Blot.


Filter By Application
IHC
(6)
IHC-F
(1)
Western Blot
(6)
All Applications
Filter By Species
Mouse
(12)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF2377 Applications Species
Y Hwang, HC Kim, EJ Shin Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice Journal of Neuroinflammation, 2022-06-11;19(1):142. 2022-06-11 [PMID: 35690821] (IHC, Mouse) IHC Mouse
Qi Zhang, Chang Liu, Rubing Shi, Shiyi Zhou, Huimin Shan, Lidong Deng et al. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke Aging and disease 6/1/2022 [PMID: 35656116]
RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard AP-1 controls the p11-dependent antidepressant response Mol. Psychiatry, 2020-05-21;0(0):. 5/21/2020 [PMID: 32439846]
B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype Cell Death Dis, 2020-05-15;11(5):377. 2020-05-15 [PMID: 32415059] (Western Blot, Mouse) Western Blot Mouse
C Zuo, H Cao, F Ding, J Zhao, Y Huang, G Li, S Huang, H Jiang, Y Jiang, F Wang Neuroprotective efficacy of different levels of high-frequency repetitive transcranial magnetic stimulation in mice with CUMS-induced depression: Involvement of the p11/BDNF/Homer1a signaling pathway J Psychiatr Res, 2020-04-05;125(0):152-163. 2020-04-05 [PMID: 32289652] (Western Blot, Mouse) Western Blot Mouse
J Yamada, C Sato, K Konno, M Watanabe, S Jinno PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy J. Neurosci., 2019-12-04;0(0):. 2019-12-04 [PMID: 31801810] (IHC, Mouse) IHC Mouse
Victoria García-Morales, Guillermo Rodríguez-Bey, Laura Gómez-Pérez, Germán Domínguez-Vías, David González-Forero, Federico Portillo et al. Sp1-regulated expression of p11 contributes to motor neuron degeneration by membrane insertion of TASK1 Nature Communications 8/22/2019 [PMID: 31439839]
D Sargin, RU Chottekala, KE Perit, V Yao, D Chu, DW Sparks, S Kalik, SK Power, OG Troyanskay, EF Schmidt, P Greengard, EK Lambe Mapping the physiological and molecular markers of stress and SSRI antidepressant treatment in S100a10 corticostriatal neurons Mol. Psychiatry, 2019-08-20;25(5):1112-1129. 2019-08-20 [PMID: 31431686] (IHC, Mouse) IHC Mouse
G Liu, Y Wang, W Zheng, H Cheng, R Zhou P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice Int. J. Biol. Sci., 2019-05-20;15(7):1383-1395. 2019-05-20 [PMID: 31337969] (Western Blot, Mouse) Western Blot Mouse
J Jin, DL Bhatti, KW Lee, L Medrihan, J Cheng, J Wei, P Zhong, Z Yan, C Kooiker, C Song, JH Ahn, GJ Obermair, A Lee, J Gresack, P Greengard, Y Kim Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior Mol. Psychiatry, 2019-02-13;0(0):. 2019-02-13 [PMID: 30760886] (Western Blot, Mouse) Western Blot Mouse
Show All 20 Publications.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

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Bioinformatics

Gene Symbol S100A10
Uniprot