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RPA14 Antibody

Images

 
Western Blot: RPA14 Antibody [NBP1-87141] - Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: RPA14 Antibody [NBP1-87141] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Western Blot: RPA14 Antibody [NBP1-87141] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RPA14 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RPA14 Protein (NBP1-87141PEP)
Publications
Read Publication using NBP1-87141.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPA14 Antibody

  • REPA3Replication factor A protein 3
  • replication protein A 14 kDa subunit
  • replication protein A3 (14kD)
  • replication protein A3, 14kDa
  • RF-A protein 3
  • RP-A p14
  • RPA14

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPA14 Antibody (NBP1-87141)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-87141 Applications Species
Foster B, Attwood M, Chong K et al. The RPA complex orchestrates K63-linked deubiquitination via ZUP1 bioRxiv 2023-01-28

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Bioinformatics

Gene Symbol RPA3