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Recombinant Mouse VEGF 120 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
494-VE-025/CF
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Recombinant Mouse VEGF 120 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-4 ng/mL.
Source
E. coli-derived mouse VEGF protein
Ala27-Arg146 (Gly117Glu & Glu118Arg)
Accession #
N-terminal Sequence
Ala27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Vegfa
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
494-VE/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VEGF 120 Protein, CF

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1‑3). It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). VEGF120 and VEGF121 lack basic heparin-binding regions and are freely diffusible (4). Mouse embryos expressing only the VEGF120 isoform do not survive to term and show defects in skeletogenesis (5). Mouse VEGF120 shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, Neuropilin-1; VEGF121 binding has also been reported (6). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (7, 8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL‑1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 10).

  1. Breier, G. et al. (1992) Development 114:521.
  2. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Zelzer, E. et al. (2002) Development 129:1893.
  6. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  7. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  8. Thurston, G. (2002) J. Anat. 200:575.
  9. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  10. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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Recombinant Mouse
VEGF 120 Protein, CF
494-VE/CF
Recombinant Mouse VEGF 120 Protein, CF
Species: Mu
Applications: Bioactivity

Publications for VEGF (494-VE/CF)(8)

We have publications tested in 3 confirmed species: Mouse, N/A, Porcine.

We have publications tested in 3 applications: Bioassay, In Vivo, Western Blot.


Filter By Application
Bioassay
(3)
In Vivo
(5)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(6)
N/A
(1)
Porcine
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 494-VE/CF Applications Species
Y Ichiyama, R Matsumoto, S Obata, O Sawada, Y Saishin, M Kakinoki, T Sawada, M Ohji Assessment of mouse VEGF neutralization by ranibizumab and aflibercept PLoS ONE, 2022-12-21;17(12):e0278951. 2022-12-21 [PMID: 36542626] (Western Blot, N/A) Western Blot N/A
J Wang, W Song, R Yang, C Li, T Wu, XB Dong, B Zhou, X Guo, J Chen, Z Liu, QC Yu, W Li, J Fu, YA Zeng Endothelial Wnts control mammary epithelial patterning via fibroblast signaling Cell Reports, 2021-03-30;34(13):108897. 2021-03-30 [PMID: 33789106] (Bioassay, Mouse) Bioassay Mouse
Lin H, Shabbir A, Molnar M, Yang J, Marion S, Canty JM, Lee T Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells. J. Cell. Physiol., 2008-08-01;216(2):458-68. 2008-08-01 [PMID: 18288639] (Bioassay, Porcine) Bioassay Porcine
Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J. Clin. Invest., 2006-05-04;116(6):1596-605. 2006-05-04 [PMID: 16680193] (In Vivo, Mouse) In Vivo Mouse
Niida S, Kondo T, Hiratsuka S, Hayashi S, Amizuka N, Noda T, Ikeda K, Shibuya M VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc. Natl. Acad. Sci. U.S.A., 2005-09-19;102(39):14016-21. 2005-09-19 [PMID: 16172397] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
Qian DZ, Wang X, Kachhap SK, Kato Y, Wei Y, Zhang L, Atadja P, Pili R The histone deacetylase inhibitor NVP-LAQ824 inhibits angiogenesis and has a greater antitumor effect in combination with the vascular endothelial growth factor receptor tyrosine kinase inhibitor PTK787/ZK222584. Cancer Res., 2004-09-15;64(18):6626-34. 2004-09-15 [PMID: 15374977] (In Vivo, Mouse) In Vivo Mouse
Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest. Ophthalmol. Vis. Sci., 2004-02-01;45(2):368-74. 2004-02-01 [PMID: 14744874] (In Vivo, Mouse) In Vivo Mouse
Banerjee K, Biswas PS, Kim B, Lee S, Rouse BT CXCR2-/- mice show enhanced susceptibility to herpetic stromal keratitis: a role for IL-6-induced neovascularization. J. Immunol., 2004-01-15;172(2):1237-45. 2004-01-15 [PMID: 14707102] (In Vivo, Mouse) In Vivo Mouse

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FAQs for VEGF (494-VE/CF). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
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Bioinformatics

Gene Symbol Vegfa
Uniprot