LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass type I membrane protein that exists in a disulfide-linked homodimer form. LYVE1 undergoes ligand-dependent internalization and recycling at the cell surface, and exhibits ligand-specific transporter trafficking between intracellular organelles and the plasma membrane. It plays a key role in the autocrine regulation of cell growth mediated by growth. LYVE1 also behaves as hyaluronan transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. A group at ImClone employed the LYVE1 antibody in their renal cell carcinoma (RCC) models to study the effects of VEGF antibody therapy and found that combinations of their DC101 antibody and sunitinib had more pleiotropic effects on blood vessel grouth1.
Immunohistochemistry: LYVE1 Antibody
Bumb’s group from the National Cancer Institute followed the trafficking of a dual-modality imaging agent in lymph nodes with the LYVE1 antibody2. In their laboratory, they developed a silica-coated ion oxide nanoparticle (SCION) for MR and fluorescence optical imaging in nude mice for use in intralymphatic drug delivery and sentinel node resections. The LYVE1 antibody was featured in a Cell publication where researchers characterized the spatially-organized innate immune response of lymph nodes upon pathogenic invasion from lymph-borne bacteria3. Additionally, very recent studies out of Klar‘s bioengineering group in Switzerland used the LYVE1 antibody to help assess their dermo-epidermal skin analogs4. These tissue-engineered models contained different pigmented derivatives that were then analyzed for blood and lymph vascularization patterns. The LYVE1 antibody was also used by this same group in their follow-up analyses of their analog skin model to demonstrate the formation of host-derived de novo neurovascular links5.
Novus Biologicals offers LYVE1 reagents for your research needs including:
